Description

S-[2-(4-Azidosalicylamido)Ethylthio]-2-Thiopyridine CAS NO 164575-82-0 is a specialized heterobifunctional crosslinking reagent featuring a photoreactive aryl azide group and a sulfhydryl-reactive 2-thiopyridyl group. This compound is critical for researchers and manufacturers requiring precise, light-activated conjugation of biomolecules, enabling the study of protein-protein interactions and the development of advanced diagnostic tools. It is primarily utilized in the fields of bioconjugation, proteomics research, and the development of affinity chromatography media.

Application

• Bioconjugation and Protein Labeling: For the site-specific, photo-induced crosslinking of peptides, proteins, and other biomolecules containing free thiol groups.
• Proteomics and Protein Interaction Studies: Used to capture and identify transient or weak protein-protein interactions in complex biological samples.
• Affinity Chromatography Ligand Immobilization: Enables the covalent attachment of thiol-containing ligands to solid supports for purification applications.
• Surface Modification and Biosensor Development: Facilitates the functionalization of sensor surfaces with specific biomolecular probes.
• Antibody-Drug Conjugate (ADC) Research: Serves as a tool compound in the development and optimization of novel linker-payload chemistries.
• Material Science: For creating novel polymeric materials with tailored biological functionalities via photochemical grafting.

Basic Information

Product Name	S-[2-(4-Azidosalicylamido)Ethylthio]-2-Thiopyridine
CAS No.	164575-82-0
Molecular Formula	C16H14N6O2S2
Molecular Weight	386.45 g/mol
Synonyms	2-Pyridyl 2-[2-(4-Azido-2-hydroxybenzamido)ethyl]disulfide; 4-Azido-2-hydroxy-N-[2-[(2-pyridinyldithio)ethyl]]benzamide; SAED; Heterobifunctional Crosslinker SAED; Photoreactive Crosslinking Reagent; N-[2-(Pyridyldithio)ethyl]-4-azidosalicylamide; Aryl Azide Crosslinker
EINECS	Contact for details

Quality Control

Our S-[2-(4-Azidosalicylamido)Ethylthio]-2-Thiopyridine is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability parameters. We adhere to ISO 9001 quality management standards, and our processes are designed to meet the rigorous demands of the life sciences industry.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and degradation. For long-term stability, desiccate to protect from moisture. Allow the container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Light yellow to yellow solid
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.