Description

5(6)-Carboxynaphthofluorescein Diacetate is a high-purity, advanced organic intermediate and fluorescent probe precursor. This compound is valued for its role in synthesizing highly sensitive and specific fluorescent labeling reagents used in bioanalytical chemistry. It is primarily required by manufacturers and research institutions in the life sciences sector developing diagnostic assays, cellular imaging probes, and advanced sensor technologies.

Application

• Fluorescent Probe Synthesis: A key precursor for manufacturing naphthofluorescein-based fluorescent dyes and labels.
• Bioconjugation Reagent: Used to create fluorescently tagged antibodies, proteins, and oligonucleotides for immunoassays and FISH (Fluorescence In Situ Hybridization).
• Cellular & Molecular Imaging: Enables the development of probes for real-time visualization of biological processes and intracellular pH sensing.
• Diagnostic Assay Development: Integral to the production of reagents for high-sensitivity ELISA, flow cytometry, and other clinical diagnostic platforms.
• Sensor Technology: Serves as a building block for chemical sensors and biosensors designed to detect specific analytes.
• Research & Development: Used in academic and industrial R&D for exploring new fluorescent markers and photophysical properties.

Basic Information

Product Name	5(6)-Carboxynaphthofluorescein Diacetate
CAS No.	163831-68-3
Molecular Formula	C33H22O9
Molecular Weight	562.52 g/mol
Synonyms	5(6)-Carboxy-2',7'-dichloro-3',6'-dihydroxy-4,5,6,7-tetrahydrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one Diacetate; 5(6)-Carboxy-2',7'-dichlorofluorescein Diacetate; Naphthofluorescein Diacetate, Carboxy-; 5(6)-Carboxy-2',7'-dichloro-3',6'-diacetoxy-4,5,6,7-tetrahydrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 5(6)-CFDA; 5(6)-Carboxynaphthofluorescein Diacetate, mixed isomers
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxynaphthofluorescein Diacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed under inert atmosphere or with desiccant after opening to maintain stability and purity.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.