Description

Sybr(R) Green I Nucleic Acid Gel Stain is a high-sensitivity, asymmetrical cyanine dye specifically designed for the detection of nucleic acids in agarose and polyacrylamide gels. Its exceptional fluorescence enhancement upon binding to double-stranded DNA makes it a critical reagent for molecular biology workflows, offering superior sensitivity compared to traditional ethidium bromide. This product is essential for research laboratories, diagnostic kit manufacturers, and biopharmaceutical companies engaged in PCR product analysis, genotyping, and DNA quantification.

Application

• Gel Electrophoresis Visualization: Primary use for staining and visualizing double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gels post-electrophoresis.
• Real-time PCR (qPCR): Used as a fluorescent intercalating dye for monitoring DNA amplification in real-time, commonly in SYBR Green-based qPCR assays.
• DNA Quantification: Employed in fluorometric assays for the sensitive quantification of DNA concentration in solution, often using microplate readers.
• Cell Viability & Apoptosis Assays: Utilized in fluorescence microscopy and flow cytometry to stain nucleic acids in fixed cells or to detect apoptotic cells (condensed chromatin).
• Nucleic Acid Detection in Capillary Electrophoresis: Applied in fragment analysis and sizing applications within capillary electrophoresis systems.
• Microbiology Research: Used for staining and enumerating bacteria in environmental or clinical samples via fluorescence techniques.

Basic Information

Product Name	Sybr(R) Green I Nucleic Acid Gel Stain
CAS No.	163795-75-3
Molecular Formula	C32H37N4S+
Molecular Weight	509.73 g/mol (for cation)
Synonyms	SYBR Green I; SYBR Green I nucleic acid gel stain; [2-[N-(3-dimethylaminopropyl)-N-propylamino]-4-[2,3-dihydro-3-methyl-(benzo-1,3-thiazol-2-yl)-methylidene]-1-phenyl-quinolinium]; 2-[4-(Dimethylamino)phenyl]ethenyl]-1-ethylquinolinium iodide (common base structure reference); SG I; SYBR® Green I (registered trademark); Fluorescent nucleic acid gel stain; Asymmetrical cyanine dye
EINECS	Contact for details

Quality Control

Our Sybr(R) Green I Nucleic Acid Gel Stain is manufactured under strict quality management systems. Each batch is subjected to rigorous analytical testing, including HPLC for purity assessment, spectrophotometry for absorbance and fluorescence verification, and functional testing in gel electrophoresis. Certificates of Analysis (COA) detailing purity, performance, and absence of contaminating nucleases are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store the concentrated stock solution in the dark at -20°C. For short-term use, aliquots can be stored at 2-8°C. Avoid repeated freeze-thaw cycles. The working solution in electrophoresis buffer should be prepared fresh or stored protected from light for limited periods. Keep away from strong acids and organic solvents.

Specification

Item	Specification
Appearance	Dark green solid or concentrated solution
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Absorbance Maximum	~497 nm (in TE buffer)
Fluorescence Emission Maximum	~520 nm (when bound to dsDNA)
Fluorescence Enhancement	>1000-fold upon binding to dsDNA
Sensitivity (Detection Limit)	< 20 pg dsDNA per band in agarose gel
Solubility	Soluble in DMSO or DMF; aqueous solutions prepared from concentrated stocks
Nuclease Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.