Description

Fluorescein 6-Maleimide is a high-purity, bifunctional labeling reagent that combines the bright fluorescence of fluorescein with the selective reactivity of a maleimide group. This compound is essential for creating stable, fluorescent conjugates with thiol-containing biomolecules, enabling precise tracking and analysis. It is a critical tool for researchers and developers in bioconjugation, proteomics, and diagnostic assay development, where reliable and specific labeling is paramount.

Application

• Bioconjugation and Protein Labeling: Selective covalent attachment to cysteine residues in proteins and antibodies for fluorescence-based studies.
• Proteomics Research: Tagging of peptides and proteins for detection, quantification, and analysis in gel electrophoresis and chromatography.
• Diagnostic Assay Development: Creation of fluorescent probes and tracers for immunoassays, lateral flow tests, and biosensors.
• Cell Biology and Imaging: Labeling of intracellular thiols or engineered proteins for fluorescence microscopy and flow cytometry.
• Pharmaceutical Analysis: Used as a derivatization agent in the analysis of thiol-containing drug compounds and metabolites.
• Material Science: Functionalization of nanoparticles and surfaces with fluorescent tags for sensing and imaging applications.

Basic Information

Product Name	Fluorescein 6-Maleimide
CAS No.	161598-40-9
Molecular Formula	C27H15NO7
Molecular Weight	465.41 g/mol
Synonyms	6-Maleimidofluorescein; Fluorescein-6-maleimide; 5(6)-Maleimidofluorescein; 6-MF; FAM-6-Maleimide; Fluorescein maleimide; 2-[6-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)-3-oxo-3H-xanthen-9-yl]benzoic acid
EINECS	Contact for details

Quality Control

Our Fluorescein 6-Maleimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, and fluorescence properties is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; desiccants are recommended for long-term storage. Keep container tightly sealed when not in use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Absorbance (λmax)	~492 nm (in suitable buffer)
Emission (λmax)	~515 nm (in suitable buffer)
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.