Description

Lucifer Yellow Ethylenediamine is a highly fluorescent, membrane-impermeant dye derivative widely used as a vital tracer in biological research. Its core value lies in its ability to form stable conjugates, enabling precise tracking and labeling of biomolecules within cellular systems. This product is essential for researchers and manufacturers in the fields of neuroscience, cell biology, and diagnostic assay development, particularly for neuronal tracing, gap junction communication studies, and fluorescence microscopy.

Application

• Neuronal Tracing and Mapping: As a classic retrograde and anterograde tracer for labeling neurons and visualizing neural pathways.
• Cell-to-Cell Communication Studies: To investigate gap junction permeability and intercellular dye coupling in live cells.
• Fluorescent Labeling & Conjugation: Serving as a reactive fluorescent tag for antibodies, peptides, dextrans, and other biomolecules.
• Microinjection & Intracellular Staining: For direct introduction into cells to monitor morphology, viability, and intracellular dynamics.
• Viability/Cytotoxicity Assays: Used as an indicator dye in assays measuring membrane integrity and cell health.
• Phagocytosis and Endocytosis Research: To track particle uptake and intracellular vesicle trafficking.
• Diagnostic Reagent Component: Incorporated into fluorescent kits and reagents for research and clinical diagnostics.
• Material Science & Sensor Development: As a fluorescent probe in the development of advanced polymeric materials and chemical sensors.

Basic Information

Product Name	Lucifer Yellow Ethylenediamine
CAS No.	161578-11-6
Molecular Formula	C₁₈H₁₈N₄O₇S₂ • 2Li (commonly as dilithium salt)
Molecular Weight	~ 472.4 g/mol (Free acid)
Synonyms	Lucifer Yellow CH; Lucifer Yellow carbohydrazide; 6-Amino-2-[(hydrazinecarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid; LYCH; Lucifer Yellow ethylenediamine, dilithium salt; Reactive Lucifer Yellow; [6-[(2-Aminoethyl)amino]-2-[(hydrazinecarbonyl)amino]-1,3-dioxo-1H-benz[de]isoquinolin-5-yl]methanesulfonic acid
EINECS	Contact for details

Quality Control

Our Lucifer Yellow Ethylenediamine is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance metrics. We ensure compliance with relevant research-grade and in-vitro diagnostic (IVD) component standards. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture. For long-term storage, consider desiccant or under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 5.0 %
Fluorescence	Emits characteristic yellow-green light under standard excitation
Solubility	Soluble in water, DMSO
Functional Activity (Labeling)	Conjugates efficiently with target biomolecules under standard conditions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.