Description

Neurodye Rh-414 is a lipophilic, styryl-based fluorescent dye widely used as a potentiometric probe for monitoring neuronal activity. Its core value proposition lies in its ability to undergo fast, reversible changes in fluorescence intensity in response to membrane potential shifts, making it an essential tool for non-invasive electrophysiological studies. This compound is primarily needed by researchers and manufacturers in the neuroscience, biotechnology, and pharmaceutical development sectors for applications such as optical imaging, high-throughput screening, and the study of ion channel function.

Application

• Optical Recording of Neuronal Activity: Primary use as a fast-response potentiometric dye for monitoring action potentials and subthreshold voltage changes in neurons and other excitable cells.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to assay the effects of pharmacological compounds on membrane potential and ion channel function.
• Brain Slice and In Vitro Electrophysiology: Visualization of network activity in brain slices, cultured neurons, and cardiac myocytes for fundamental neuroscience and cardiology research.
• Fluorescence Imaging Microscopy: Used in conjunction with confocal or two-photon microscopy for high-resolution, spatially resolved mapping of electrical activity.
• Development of Biosensors: Serves as a key component in the design and calibration of optical biosensors for physiological monitoring.
• Study of Mitochondrial Membrane Potential: Alternative application for assessing changes in the mitochondrial membrane potential in cell biology studies.

Basic Information

Item	Detail
Product Name	Neurodye Rh-414
CAS No.	161433-30-3
Molecular Formula	C27H29N2O3+
Molecular Weight	429.53 g/mol
Synonyms	RH 414; RH414; Neuro-Dye RH-414; N-(3-Triethylammoniumpropyl)-4-(4-(4-(Diethylamino)phenyl)butadienyl)pyridinium Dibromide; 4-{4-[4-(Diethylamino)phenyl]-1,3-butadienyl}-1-[3-(triethylammonio)propyl]pyridinium Dibromide; Potentiometric Probe RH-414; Voltage-sensitive dye RH414; JPW 1114; JPW-1114
EINECS	Contact for details

Quality Control

Our Neurodye Rh-414 is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot is characterized by advanced analytical techniques, including HPLC for purity assessment and spectroscopic methods for identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance-related specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). Desiccate if necessary for long-term storage. Handle and store according to good laboratory practices for light-sensitive biochemical reagents.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms to reference standard
Identification (UV-Vis)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Fluorescence Properties	Ex/Em maxima conform to typical range
Functional Performance	Suitable for optical recording applications

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.