Description

6-Aminotetramethylrhodamine is a high-purity, amine-reactive derivative of the classic rhodamine fluorophore, widely recognized for its bright orange-red fluorescence and excellent photostability. This compound is a critical building block for synthesizing fluorescent probes, labels, and conjugates used in advanced bioanalytical and diagnostic applications. It is essential for researchers and manufacturers in the fields of life sciences, diagnostics, and flow cytometry who require reliable, high-performance fluorescent tags for labeling antibodies, proteins, and oligonucleotides.

Application

• Fluorescent Labeling Reagent: Primary application for covalent conjugation to biomolecules (antibodies, proteins, peptides) via its amine-reactive succinimidyl ester (NHS ester) form.
• Flow Cytometry & Immunofluorescence: Used to create bright, photostable antibody conjugates for cell surface marker detection and intracellular staining.
• Fluorescence In Situ Hybridization (FISH): Serves as a fluorescent tag for nucleic acid probes in genetic testing and cytogenetics.
• Bioconjugation & Probe Synthesis: Key intermediate in the custom synthesis of fluorescent sensors, enzyme substrates, and molecular beacons.
• Microscopy & Imaging: Provides a robust fluorescent signal for fixed-cell imaging, tissue section analysis, and super-resolution microscopy techniques.
• Diagnostic Assay Development: Incorporated into lateral flow assays, ELISA, and other diagnostic platforms as a detection label.

Basic Information

Item	Detail
Product Name	6-Aminotetramethylrhodamine
CAS No.	159435-10-6
Molecular Formula	C25H23N3O3
Molecular Weight	413.47 g/mol
Synonyms	6-TAMRA amine; 6-Carboxytetramethylrhodamine amine; 5(6)-TAMRA, SE; Tetramethylrhodamine-5(6)-isothiocyanate precursor; 6-Amino-3',6'-bis(dimethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; Rhodamine 6G amine derivative; Xanthylium, 9-(2-carboxyphenyl)-3,6-bis(dimethylamino)-, chloride, 6-amino derivative
EINECS	Contact for details

Quality Control

Our 6-Aminotetramethylrhodamine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A detailed Certificate of Analysis (COA) providing batch-specific data is supplied with every order.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area %)
Absorption (λmax)	~525 - 555 nm
Emission (λmax)	~555 - 580 nm
Water Content (KF)	≤ 5.0 %
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.