Description

6-Chloroacetamidotetramethylrhodamine is a chemically reactive derivative of the widely used tetramethylrhodamine (TAMRA) fluorophore. This compound matters for its role as a critical labeling reagent, enabling the covalent attachment of the bright, photostable TAMRA dye to biomolecules for advanced detection and imaging. It is primarily needed by researchers and manufacturers in the life sciences, particularly for applications in fluorescence microscopy, flow cytometry, and the development of diagnostic assays and probes.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to proteins, peptides, antibodies, and oligonucleotides for detection and visualization.
• Probe and Sensor Synthesis: A key building block in the development of fluorescent chemical probes and biosensors for monitoring biological processes.
• Cell and Tissue Imaging: Used to prepare labeled ligands or tracers for fluorescence microscopy (e.g., confocal, super-resolution) and histology.
• Flow Cytometry Reagents: Manufacture of fluorescently-tagged antibodies and other detection reagents for multiparameter cell analysis.
• Diagnostic Assay Development: Labeling component for in vitro diagnostic (IVD) kits, including ELISA and lateral flow assays.
• Nucleic Acid Analysis: Labeling of primers, probes, and nucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.

Basic Information

Product Name	6-Chloroacetamidotetramethylrhodamine
CAS No.	159435-08-2
Molecular Formula	C27H24ClN3O4
Molecular Weight	489.95 g/mol
Synonyms	6-TAMRA cadaverine; 6-Carboxytetramethylrhodamine cadaverine; 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester (isomer); 6-TAMRA, SE; 6-TAMRA NHS ester; TAMRA-X, SE; 6-Chloroacetamido-TAMRA; N-(5-Carboxypentyl)-4',5'-dichloro-2',7'-dimethoxy-5(6)-carboxyfluorescein (mixture of isomers)
EINECS	Contact for details

Quality Control

Our 6-Chloroacetamidotetramethylrhodamine is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); desiccate upon opening and seal tightly after each use to maintain stability and performance.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~546 nm
Emission Maximum	~579 nm
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.