Description

Iodohoechst 33258 is a fluorescent dye and a derivative of the classic DNA-binding stain Hoechst 33258, where iodine substitution enhances its photophysical properties. This compound is valued for its high specificity in binding to the minor groove of double-stranded DNA, making it a critical tool for nucleic acid detection and quantification. It is primarily needed by researchers and manufacturers in the life sciences, molecular diagnostics, and biotechnology sectors for applications in flow cytometry, fluorescence microscopy, and high-throughput screening.

Application

• Nucleic Acid Staining & Quantification: Used for the sensitive detection and quantification of double-stranded DNA in solution, including in microplate assays.
• Flow Cytometry: Employed as a cell-permeant stain for DNA content analysis and cell cycle studies.
• Fluorescence Microscopy: Serves as a specific nuclear counterstain in fixed cells for imaging applications.
• Biochemical Research: Utilized in studies of DNA-protein interactions and chromatin structure due to its specific minor groove binding.
• Diagnostic Assay Development: A component in the development of fluorescent-based diagnostic kits and high-content screening platforms.
• Quality Control in Biotechnology: Applied in processes to assess DNA purity and concentration in biomanufacturing.

Basic Information

Product Name	Iodohoechst 33258
CAS No.	158013-43-5
Molecular Formula	C25H24I3N6O
Molecular Weight	814.11 g/mol
Synonyms	Iodo-Hoechst 33258; Bisbenzimide Iodide Derivative; 2'-(4-Iodophenyl)-5'-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole triiodide; Hoechst 33258 Iodide; Iodinated Hoechst 33258; DNA Stain Iodohoechst; Fluorescent DNA Probe Iodohoechst
EINECS	Contact for details

Quality Control

Our Iodohoechst 33258 is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification (UV-Vis, IR), and functional performance validation in standard DNA-binding assays. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. For specific purity grades or custom quality agreements, please contact our technical team.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, keep desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (UV-Vis)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence Performance	Passes functional test in standard DNA assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.