Description

Iodohoechst 33258 is a fluorescent dye and a derivative of the well-known DNA-binding agent Hoechst 33258, where iodine substitution enhances specific photophysical properties. This compound is critical for advanced research applications requiring high-affinity nucleic acid staining with modified spectral characteristics. It is primarily needed by biotechnology, pharmaceutical, and life science research laboratories for fluorescence microscopy, flow cytometry, and other bioanalytical techniques.

Application

• Nucleic Acid Staining & Visualization: Used as a fluorescent probe for staining DNA in fixed cells and chromosomes for fluorescence microscopy.
• Flow Cytometry Analysis: Employed in cell cycle analysis and ploidy determination due to its specific binding to AT-rich regions of DNA.
• Biochemical Research: Serves as a tool in studies of DNA-protein interactions, chromatin structure, and nucleic acid quantification assays.
• Photophysical Studies: Utilized in research investigating halogenated dye derivatives and their enhanced photostability or emission properties.
• Diagnostic Development: Acts as a potential component in the development of diagnostic kits and reagents for cytogenetic analysis.

Basic Information

Product Name	Iodohoechst 33258
CAS No.	158013-41-3
Molecular Formula	C25H24I3N6O
Molecular Weight	821.11 g/mol
Synonyms	Iodo-Hoechst 33258; 2'-(4-Iodophenyl)-5'-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole triiodide; Hoechst 33258 iodo derivative; Bisbenzimide Iodo derivative; Iodinated Hoechst 33258; DNA Stain Iodo-Hoechst; 4-Iodo-Hoechst 33258
EINECS	Contact for details

Quality Control

Our Iodohoechst 33258 is produced under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Solubility Test	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.