Description

Di-8-Anepps is a highly specialized voltage-sensitive fluorescent dye belonging to the ANEPPS family of potentiometric probes. This compound is critical for non-invasive, real-time optical monitoring of transmembrane potential changes in excitable cells and tissues. Researchers in neuroscience, cardiology, and cellular biophysics rely on Di-8-ANEPPS for high-resolution electrophysiological imaging, making it an essential tool for studying action potentials, ion channel function, and drug effects on membrane dynamics.

Application

• Optical Electrophysiology: Primary use for fast, sensitive detection of membrane potential changes in neurons, cardiac myocytes, and other excitable cells.
• Cardiac Research: Mapping action potential propagation and arrhythmia mechanisms in heart tissue and stem-cell derived cardiomyocytes.
• Neuroscience & Neuropharmacology: Visualizing neuronal network activity and screening compounds that modulate neuronal excitability and synaptic function.
• Cellular Biophysics: Investigating fundamental properties of cell membranes, including lipid domain organization and its effect on electrical signaling.
• High-Throughput Screening (HTS): Enabling fluorescence-based assays in drug discovery pipelines for ion channel targets and cardiotoxicity testing.
• Combined Imaging Techniques: Used alongside calcium indicators or other probes for multimodal functional imaging studies.

Basic Information

Item	Detail
Product Name	Di-8-Anepps
CAS No.	157134-53-7
Molecular Formula	C30H39N2O3S2
Molecular Weight	539.77 g/mol
Synonyms	Di-8-ANEPPS; JPW 1114; 4-(2-(6-(Dioctylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)pyridinium, inner salt; 1-(3-Sulfopropyl)-4-[β-[2-(dioctylamino)-6-naphthyl]vinyl]pyridinium Betaine; ANEPPS dye; Voltage-sensitive fluorescent dye; Potentiometric probe
EINECS	Contact for details

Quality Control

Our Di-8-ANEPPS is produced and tested to meet the exacting standards required for sensitive research applications. Each batch is subjected to rigorous analytical characterization, including HPLC for purity and spectral analysis (UV-Vis, Fluorescence) to confirm correct photophysical properties. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance specifications is provided with every shipment to ensure traceability and reproducibility for your experiments.

Storage

Preserve in a tightly closed container, protected from light. Store the product at -20°C or below under desiccated conditions (e.g., in a sealed container with desiccant) due to its hygroscopic and light-sensitive nature. For long-term stability, solutions should be prepared in anhydrous DMSO, aliquoted, and stored at ≤ -20°C, protected from light and moisture. Allow the product to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 95% (Area %)
Absorption Max (λmax)	~488 nm (in methanol)
Emission Max (λem)	~613 nm (in methanol)
Extinction Coefficient (ε)	~57,000 cm-1M-1 (at λmax)
Solubility	Soluble in DMSO, DMF, ethanol; sparingly soluble in aqueous buffers
Water Content (KF)	< 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.