Description

Hoechst 33342 Analog 2 (Trihydrochloride) is a cell-permeable, blue-fluorescent nuclear stain widely used as a vital dye in life science research. Its core value lies in its ability to selectively bind to DNA in live and fixed cells, making it an essential tool for cell cycle analysis, viability assays, and chromosome staining. This analog is particularly sought after by researchers in molecular biology, cytology, and pharmacology for applications in flow cytometry, fluorescence microscopy, and high-content screening. The trihydrochloride salt form enhances its solubility in aqueous buffers, facilitating its use in complex biological assays.

Application

• Live-Cell Nuclear Staining: For real-time visualization of nuclei in viable cells using fluorescence microscopy.
• Flow Cytometry Analysis: Used in DNA content and cell cycle analysis to quantify ploidy and identify different phases of the cell cycle.
• Cell Viability Assays: Employed to distinguish between live and dead cells based on differential staining characteristics.
• Chromosome Banding: Aids in cytogenetic studies for chromosome identification and karyotyping.
• High-Content Screening (HCS): Serves as a nuclear counterstain in automated imaging platforms for drug discovery and toxicology studies.
• Apoptosis Detection: Used in combination with other markers to study programmed cell death and nuclear morphology changes.

Basic Information

Product Name	Hoechst 33342 Analog 2 (Trihydrochloride)
CAS No.	155815-98-8
Molecular Formula	C27H30N6O · 3HCl
Molecular Weight	561.94 g/mol (free base)
Synonyms	Bisbenzimide H 33342 analog; HOE 33342 Analog; Trihydrochloride salt of Hoechst 33342 derivative; Nuclear stain 33342 analog; Cell-permeable blue fluorescent DNA dye; Vital DNA stain; CAS 155815-98-8; 2'-[4-Ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride
EINECS	Contact for details

Quality Control

Our Hoechst 33342 Analog 2 (Trihydrochloride) is produced under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, identity confirmation by spectroscopic methods, and validation of functional performance in biological assays. Certificates of Analysis (COA) detailing all specifications and test results are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, desiccate at -20°C.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO
Fluorescence Properties	Ex/Em ~350/461 nm (bound to DNA)
Functional Activity	Suitable for cell staining

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.