Description

Tetramethylrhodamine-5-Maleimide is a high-purity, reactive fluorescent dye derivative used for site-specific labeling of thiol groups in proteins, peptides, and other biomolecules. Its core value lies in providing a bright, photostable red-orange fluorescent tag, enabling sensitive detection and analysis in complex biological systems. This reagent is essential for researchers and manufacturers in the fields of bioconjugation, proteomics, bioassay development, and diagnostic kit production, where precise and stable fluorescent labeling is required.

Application

• Protein & Antibody Labeling: Covalent conjugation to cysteine residues for fluorescence-activated cell sorting (FACS), fluorescence microscopy, and Western blotting.
• Bioconjugation & Probe Synthesis: Creating fluorescently labeled peptides, oligonucleotides, or small molecules for target detection and binding studies.
• Diagnostic Assay Development: Used as a critical component in the manufacture of fluorescent immunoassays (FLIA) and other in vitro diagnostic (IVD) reagents.
• Biomolecular Tracking: Real-time visualization and tracking of biomolecules in live-cell or fixed-cell imaging applications.
• Pharmaceutical Research: Tool compound for studying protein-protein interactions, drug targeting, and pharmacokinetics via fluorescence.
• Material Science: Functionalization of surfaces or nanoparticles with fluorescent markers for sensor development.

Basic Information

Product Name	Tetramethylrhodamine-5-Maleimide
CAS No.	154480-30-5
Molecular Formula	C26H21N3O6
Molecular Weight	471.46 g/mol
Synonyms	5-Tetramethylrhodamine maleimide; TMR maleimide; 5-TMR maleimide; Tetramethylrhodamine-5-maleimide; 5-(and-6)-Tetramethylrhodamine N-(1-maleimidyl); 6-TMR maleimide (isomer mixture); TAMRA-5-maleimide; Rhodamine-based maleimide dye
EINECS	Contact for details

Quality Control

Our Tetramethylrhodamine-5-Maleimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for life science research and in vitro diagnostic applications. Certificates of Analysis (COA) detailing purity, absorbance/fluorescence properties, and performance data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥95.0%
Extinction Coefficient (ε)	~95,000 cm⁻¹M⁻¹ (at ~543 nm)
Emission Maximum	~567 nm
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	<1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.