Description

Alamar Blue is a water-soluble, cell-permeable, and non-toxic redox indicator widely used as a vital dye for assessing cell viability and proliferation. Its core value lies in its ability to provide a simple, rapid, and quantitative colorimetric or fluorometric readout of metabolic activity. This makes it an indispensable tool for researchers and quality control professionals in the biotechnology, pharmaceutical, and academic research sectors who require reliable cytotoxicity screening and cell health monitoring.

Application

• Cell Viability and Proliferation Assays: Primary application for screening drug candidates, evaluating cytotoxicity, and monitoring cell growth in vitro.
• Microbial Cell Viability Testing: Used in microbiology for assessing bacterial and fungal metabolic activity and susceptibility to antimicrobial agents.
• High-Throughput Screening (HTS): Ideal for automated screening platforms in drug discovery due to its homogenous format and simple protocol.
• Stem Cell Research: Monitoring the metabolic activity and health of stem cell cultures without inducing toxicity.
• Cancer Research: Evaluating the efficacy of chemotherapeutic agents and radiation treatments on cancer cell lines.
• Biocompatibility Testing: Assessing the cytotoxicity of biomaterials, medical devices, and industrial chemicals.

Basic Information

Product Name	Alamar Blue
CAS No.	153796-08-8
Molecular Formula	C12H7NO4
Molecular Weight	229.19 g/mol
Synonyms	Resazurin; 7-Hydroxy-3H-phenoxazin-3-one 10-oxide; Diazo-resorcinol; Azoresorcin; NSC 47774; Dioxotetrahydrooxophenoxazine; AlamarBlue™ Cell Viability Reagent
EINECS	Contact for details

Quality Control

Our Alamar Blue is manufactured under strict quality management systems to ensure batch-to-batch consistency and performance reliability for critical research applications. Each lot is subjected to rigorous analytical testing, including identity confirmation, purity assessment, and functional performance validation in cell-based assays. A comprehensive Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) in a dry environment. The product is light-sensitive; prolonged exposure to light should be avoided to maintain stability.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Solubility (in Water)	Clear, dark blue solution
Functional Performance	Passes cell viability assay validation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.