Description

6-Carboxytetramethylrhodamine n-Hydroxysuccinimide Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used in the preparation of biological probes. Its core value lies in its ability to form stable amide bonds with primary amines, facilitating the covalent tagging of proteins, peptides, and oligonucleotides with the bright, photostable TAMRA fluorophore. This compound is essential for researchers and manufacturers in life sciences, diagnostics, and pharmaceutical development who require reliable, high-performance conjugates for fluorescence-based assays, imaging, and detection.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for use in immunofluorescence (IF), flow cytometry (FACS), and fluorescence in situ hybridization (FISH).
• Probe Synthesis for Diagnostics: Key reagent in the development of fluorescent probes and kits for clinical diagnostics, including in vitro diagnostics (IVD) and point-of-care testing.
• Bioconjugation for Drug Discovery: Used to label drug candidates, ligands, or nanoparticles to study binding affinity, cellular uptake, and pharmacokinetics via fluorescence detection.
• Microarray and Biochip Fabrication: Immobilization of labeled biomolecules on solid surfaces for high-throughput screening and genomic/proteomic analysis.
• FRET (Förster Resonance Energy Transfer) Studies: Acts as an efficient acceptor or donor dye in FRET pairs for investigating molecular interactions and conformational changes.
• Cell Tracing and Imaging: Labeling of cells or subcellular components for long-term tracking and high-resolution microscopy, including confocal and super-resolution imaging.

Basic Information

Product Name	6-Carboxytetramethylrhodamine n-Hydroxysuccinimide Ester
CAS No.	150810-68-7
Molecular Formula	C32H31N3O7
Molecular Weight	569.61 g/mol
Synonyms	6-TAMRA NHS Ester; 6-Carboxy-tetramethylrhodamine N-succinimidyl ester; 6-TAMRA, SE; 5(6)-TAMRA NHS ester; Tetramethylrhodamine-6-isothiocyanate isomer; 6-Carboxytetramethylrhodamine succinimidyl ester; TAMRA 6 NHS ester; Rhodamine 6G derivative NHS ester
EINECS	Contact for details

Quality Control

Our 6-Carboxytetramethylrhodamine NHS Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, spectrophotometry for dye content and labeling efficiency verification, and identity confirmation via mass spectrometry (MS). Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We support compliance with relevant quality frameworks for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Dye Content (Absorbance)	≥ 90%
Extinction Coefficient (ε)	~90,000 cm⁻¹M⁻¹ at 555 nm
Fluorescence Emission	~580 nm (max)
Solubility	DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.