Description

3'',6''-O,O''-Diacetyl-4'',5''-Dimethyl-5(6)-(n-Succinimidyloxycarbonyl)-Fluores is a highly reactive, amine-reactive derivative of a fluorescein-based dye, designed for efficient labeling of biomolecules. This compound is critical for researchers and manufacturers requiring stable, fluorescent conjugates with high quantum yield for sensitive detection. It is primarily used in the development of diagnostic assays, fluorescence microscopy, and flow cytometry applications within the biotechnology and pharmaceutical industries.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amines (lysine residues) for creating detection reagents in immunoassays (ELISA, Western Blot).
• Nucleic Acid Probe Synthesis: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Cell and Tissue Staining: Preparation of fluorescent tracers and tags for live-cell imaging and histology.
• Diagnostic Kit Manufacturing: A key raw material in the production of lateral flow assays and other point-of-care diagnostic devices.
• Bioconjugation Research: Used in R&D for developing novel fluorescent sensors and molecular probes.
• Flow Cytometry Reagent Development: Creating bright and photostable antibody-dye conjugates for cell surface marker analysis.

Basic Information

Product Name	3'',6''-O,O''-Diacetyl-4'',5''-Dimethyl-5(6)-(n-Succinimidyloxycarbonyl)-Fluores
CAS No.	150347-61-8
Molecular Formula	C31H24N2O10
Molecular Weight	584.53 g/mol
Synonyms	5(6)-FAM, SE; 5(6)-Carboxyfluorescein N-succinimidyl ester, diacetate; 6-FAM, SE diacetate; Fluorescein-5(6)-isothiocyanate analogue, NHS ester; 4',5'-Dimethylfluorescein-5(6)-NHS ester, diacetate; 3',6'-Diacetyl-4',5'-dimethylfluorescein-5(6)-NHS ester; 5(6)-Carboxy-4',5'-dimethyl-3',6'-diacetylfluorescein N-succinimidyl ester
EINECS	Contact for details

Quality Control

Our 3'',6''-O,O''-Diacetyl-4'',5''-Dimethyl-5(6)-(n-Succinimidyloxycarbonyl)-Fluores is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive life science applications. Each lot is analyzed via HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in conjugation is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Single Isomer Content (5- or 6- isomer)	≥ 90.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile
Functional Performance	Conjugation efficiency ≥ 85% (typical)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.