Description

5-Carboxytetramethylrhodamine is a high-purity, functionalized derivative of the classic rhodamine dye family, distinguished by its carboxylic acid group. This reactive moiety is critical for covalent conjugation to biomolecules, enabling the creation of bright, photostable fluorescent probes and labels. This compound is essential for researchers and manufacturers in the life sciences and diagnostics sectors who require reliable, high-performance fluorescent tags for advanced applications such as flow cytometry, immunofluorescence, and nucleic acid detection.

Application

• Fluorescent Labeling Reagent: Primary use as an amine-reactive succinimidyl ester (NHS ester) precursor for labeling antibodies, proteins, peptides, and oligonucleotides.
• Bioconjugation & Probe Synthesis: Key building block for synthesizing custom fluorescent probes, molecular beacons, and in-situ hybridization (FISH) kits.
• Flow Cytometry & Cell Analysis: Used to create bright fluorophore-conjugated antibodies and other detection reagents for multiparameter cell sorting and analysis.
• Fluorescence Microscopy & Imaging: Provides a red-emitting fluorophore (ex/em ~546/576 nm) for high-contrast cellular and tissue imaging in immunofluorescence and live-cell studies.
• Diagnostic Assay Development: Integral component in the development of ELISA, lateral flow assays, and other clinical diagnostic tests requiring sensitive fluorescent detection.
• Materials Science: Used in the fabrication of fluorescent sensors, nanoparticles, and advanced polymeric materials for research and industrial sensing applications.

Basic Information

Item	Detail
Product Name	5-Carboxytetramethylrhodamine
CAS No.	150322-05-7
Molecular Formula	C25H21NO6
Molecular Weight	431.44 g/mol
Synonyms	5-Carboxy TAMRA; 5-TAMRA acid; 6-Carboxytetramethylrhodamine (positional isomer); TAMRA-COOH; 5(6)-Carboxytetramethylrhodamine (mixed isomers); 4,7-Dichloro-5(or 6)-carboxyrhodamine 110; Rhodamine B 5-carboxylic acid; X-rhodamine-5-carboxylic acid
EINECS	Contact for details

Quality Control

Our 5-Carboxytetramethylrhodamine is produced under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and identity confirmation. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect performance.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorbance (λmax)	546 ± 5 nm
Emission (λmax)	576 ± 5 nm
Extinction Coefficient (ε)	≥ 90,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 3.0%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.