Description

5-Maleimidofluorescein Diacetate is a bifunctional fluorescent labeling reagent that combines a fluorescein-based fluorophore with a maleimide reactive group. This compound is critical for creating stable, fluorescent conjugates with thiol-containing biomolecules, enabling high-sensitivity detection and analysis. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, proteomics, and diagnostic assay development.

Application

• Bioconjugation and Protein Labeling: Covalently attaches to cysteine residues in proteins and peptides for fluorescence tagging.
• Antibody-Drug Conjugate (ADC) Research: Serves as a model linker-payload or fluorescent tracer in ADC development and characterization.
• Fluorescence-Based Assays: Used in ELISA, Western blotting, and other immunoassays for signal generation and detection.
• Cell Imaging and Tracking: Labels thiol groups on cell surface proteins or internal components for microscopy and flow cytometry.
• Diagnostic Kit Development: A key component in the manufacture of fluorescent diagnostic reagents and test strips.
• Biomolecular Interaction Studies: Facilitates Förster Resonance Energy Transfer (FRET) and other proximity-based assays.

Basic Information

Product Name	5-Maleimidofluorescein Diacetate
CAS No.	150322-01-3
Molecular Formula	C31H21NO10
Molecular Weight	567.50 g/mol
Synonyms	5-Maleimidofluorescein Diacetate; 5-MF Diacetate; Fluorescein-5-maleimide Diacetate; 5-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic Acid Diacetate; 5-Maleimidomethylfluorescein Diacetate (common alias); MFDA; 5-MF-DA; 5-Maleimido-Fluorescein Di-Acetate
EINECS	Contact for details

Quality Control

Our 5-Maleimidofluorescein Diacetate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, appearance, and specific performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange-yellow solid
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Solubility	Soluble in DMSO, DMF
Extinction Coefficient	~80,000 cm⁻¹M⁻¹ (at 494 nm, pH 9)
Fluorescence Emission	~518 nm (pH 9)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.