Description

5-CFDA N-Succinimidyl Ester is a vital fluorescent labeling reagent used for the covalent tagging of biomolecules, particularly proteins and antibodies. Its core value lies in its ability to impart a bright, stable green fluorescence upon hydrolysis by intracellular esterases, enabling high-sensitivity detection and tracking. This compound is essential for researchers and manufacturers in the fields of cell biology, immunology, and diagnostic assay development, where precise cellular labeling and flow cytometry are required.

Application

• Fluorescent Cell Labeling and Tracking: Used as a cell-permeant tracer to monitor cell proliferation, viability, and migration in live-cell imaging and flow cytometry.
• Antibody and Protein Conjugation: Serves as a key reagent for covalently attaching a fluorescent tag to antibodies, proteins, and peptides for use in immunofluorescence (IF) and immunohistochemistry (IHC).
• Diagnostic Kit Manufacturing: Incorporated into the production of fluorescent-based diagnostic kits and reagents for clinical and research applications.
• Intracellular Esterase Activity Assays: Functions as a substrate to measure esterase activity within living cells, a common indicator of cell health.
• Phagocytosis and Endocytosis Studies: Employed to label particles or microorganisms for quantitative analysis of phagocytic activity by immune cells.
• High-Content Screening (HCS): Utilized in automated imaging platforms for drug discovery and toxicology studies that require fluorescent cellular readouts.

Basic Information

Product Name	5-CFDA N-Succinimidyl Ester
CAS No.	150206-05-6
Molecular Formula	C25H21F3N2O8
Molecular Weight	534.44 g/mol
Synonyms	5-Carboxyfluorescein Diacetate N-Succinimidyl Ester; 5-CFSE; 5-CFDA, SE; CFDA-SE; CFSE; 5-(and-6)-Carboxyfluorescein Diacetate Succinimidyl Ester; CellTrace™ CFSE Cell Proliferation Kit (related commercial product); Fluorescein Diacetate, 5-Carboxy-, Succinimidyl Ester
EINECS	Contact for details

Quality Control

Every batch of 5-CFDA N-Succinimidyl Ester is subjected to rigorous analytical testing to ensure high purity and consistent performance. Our quality assurance protocols include identity confirmation by spectroscopic methods and purity assessment by HPLC. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment to support your research and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.