Description

Fura Red CAS NO 149732-62-7 is a high-purity, cell-permeant fluorescent calcium indicator widely used in biological research. This compound is essential for the quantitative measurement of intracellular calcium ion (Ca²⁺) concentrations in live cells using rationetric fluorescence microscopy. It is a critical tool for researchers and professionals in neuroscience, pharmacology, and cell biology who require precise, dynamic imaging of cellular signaling events. Its spectral properties allow for dual-excitation rationetric measurements, providing superior accuracy and reduced artifacts compared to single-wavelength indicators.

Application

• Intracellular Calcium Imaging: Primary use for real-time, rationetric visualization of Ca²⁺ flux in neurons, cardiomyocytes, and other cell types.
• Drug Discovery & Screening: Employed in high-throughput assays to evaluate compound effects on cellular calcium signaling pathways.
• Neurophysiological Research: Critical for studying synaptic transmission, neuronal excitability, and calcium-dependent neurotransmitter release.
• Cardiovascular Research: Used to monitor calcium transients and excitation-contraction coupling in cardiac muscle cells.
• Cell Signaling Studies: Facilitates investigation of GPCR (G-protein-coupled receptor) activation, ion channel function, and second messenger systems.
• Fluorescence Microscopy & Flow Cytometry: Serves as a key fluorescent probe for advanced imaging techniques and cell population analysis.

Basic Information

Product Name	Fura Red
CAS No.	149732-62-7
Molecular Formula	C₃₀H₂₈Cl₂N₂O₁₃
Molecular Weight	695.46 g/mol
Synonyms	Fura Red, AM; Fura Red, acetoxymethyl ester; Fura-2, 5-(and-6)-Carboxynaphthofluorescein; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, acetoxymethyl ester; Calcium indicator Fura Red; Fura Red fluorescent dye.
EINECS	Contact for details

Quality Control

Our Fura Red is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, spectral validation, and functional performance testing in cellular assays. A detailed Certificate of Analysis (COA) providing identity, purity, and absorbance/fluorescence characteristics is supplied with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Absorbance (Ethanol)	λmax ~ 472 nm, 530 nm
Functional Test (in cells)	Conforms to performance criteria

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.