Description

Calcein-Am CAS NO 148504-34-1 is a cell-permeant, non-fluorescent acetoxymethyl (AM) ester derivative of the fluorescent calcium indicator Calcein. Upon entering live cells, intracellular esterases cleave the AM groups, trapping the highly fluorescent Calcein inside, making it an essential tool for long-term cell viability and cytotoxicity assays. This vital dye is primarily used by researchers and quality control laboratories in the biotechnology, pharmaceutical, and life sciences industries for applications ranging from basic cell biology to high-throughput drug screening.

Application

• Live/Dead Cell Viability Assays: Standardized method for distinguishing between viable (fluorescent green) and non-viable cells.
• Cytotoxicity Testing: Used in pharmaceutical development to screen compound libraries for toxic effects on cultured cells.
• Cell Tracing and Proliferation Studies: Long-term tracking of cell populations due to its stable fluorescence and low leakage.
• Multidrug Resistance (MDR) Research: Functional assays to study P-glycoprotein and other efflux pump activity.
• Membrane Integrity Assessment: Indicator of plasma membrane damage in various experimental models.
• High-Content Screening (HCS): Compatible with automated imaging systems for large-scale biological analysis.

Basic Information

Item	Detail
Product Name	Calcein-Am
CAS No.	148504-34-1
Molecular Formula	C46H46N2O23
Molecular Weight	994.87 g/mol
Synonyms	Calcein AM; Calcein Acetoxymethyl Ester; 3',6'-Bis(acetyloxy)-5(6)-carboxyfluorescein diacetoxymethyl ester; 2',7'-Bis-(N,N-bis(carboxymethyl)amino)methyl]fluorescein tetraacetoxymethyl ester; Fluorexon AM; NSC 113927; Calcein, AM; Cell-permeant Calcein
EINECS	Contact for details

Quality Control

Our Calcein-Am is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot is characterized by advanced analytical techniques including HPLC for purity and functional testing in cell-based assays. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Functional Activity (Cell Assay)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.