Description

5(6)-Carboxy-2',7'-Dichlorofluorescein Diacetate n-Succinimidyl Ester is a highly reactive, amine-reactive fluorescent labeling reagent derived from dichlorofluorescein. This compound matters for researchers and industrial developers who require high-sensitivity detection and stable covalent conjugation to biomolecules like proteins, peptides, and antibodies. It is primarily needed by professionals in biotechnology, pharmaceutical R&D, and diagnostic assay development for creating advanced fluorescent probes and detection systems.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to primary amines (-NH2) on proteins, antibodies, and peptides for fluorescence-based assays.
• Cell Biology & Imaging: Preparation of fluorescent probes for intracellular labeling, tracking, and visualization in live or fixed cells.
• Diagnostic Assay Development: Key reagent in the manufacture of fluorescent immunoassays (FIAs) and enzyme-linked immunosorbent assays (ELISAs) for clinical diagnostics.
• Flow Cytometry & Microscopy: Creation of specific fluorescent tags for cell sorting (FACS) and high-resolution imaging applications.
• Bioconjugation & Probe Synthesis: Used in chemical biology for synthesizing advanced molecular probes to study protein interactions and localization.
• Research & Development: A critical tool in academic and industrial R&D for developing new detection methodologies in biochemistry and molecular biology.

Basic Information

Product Name	5(6)-Carboxy-2',7'-Dichlorofluorescein Diacetate n-Succinimidyl Ester
CAS No.	147265-60-9
Molecular Formula	C33H21Cl2NO12
Molecular Weight	694.42 g/mol
Synonyms	5(6)-CDCFDA SE; 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester; 2',7'-Dichloro-5(6)-carboxyfluorescein diacetate N-succinimidyl ester; 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-hydroxysuccinimide ester; DCFDA SE; 5(6)-Carboxy-DCFDA SE; Dichlorofluorescein diacetate NHS ester; 5(6)-CDCF Diacetate Succinimidyl Ester
EINECS	Contact for details

Quality Control

Every batch of our 5(6)-Carboxy-2',7'-Dichlorofluorescein Diacetate n-Succinimidyl Ester undergoes rigorous analytical testing to ensure high purity, consistent reactivity, and batch-to-batch reproducibility critical for sensitive research and diagnostic applications. Our quality protocols are designed to meet the stringent requirements of GMP/GLP environments. Comprehensive Certificates of Analysis (COA) detailing purity, identity, and performance characteristics are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere (argon or nitrogen) and allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder or crystalline solid
Identification (HPLC/UV)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile
Extinction Coefficient	Contact for details
Fluorescence Properties	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.