Description

Everfluor Fl, Se [4,4-Difluoro-5,7-Dimethyl-4-Bora-3A,4A-Diaza-S-Indacene-3-Propionic Acid, Succinimidyl Ester] is a high-purity, reactive succinimidyl ester derivative of the renowned BODIPY fluorophore, designed for efficient labeling of biomolecules. This compound is critical for researchers and industrial developers requiring bright, photostable fluorescent probes with excellent chemical stability for advanced detection and imaging applications. It is primarily utilized in the life sciences, diagnostics, and materials science sectors for creating conjugates with proteins, peptides, and other amine-containing molecules.

Application

• Bioconjugation & Probe Development: Primary application for covalent labeling of antibodies, proteins, peptides, and oligonucleotides via their primary amine groups.
• Fluorescence Microscopy & Imaging: Used to create highly fluorescent conjugates for cellular and tissue imaging, including confocal and super-resolution microscopy.
• Flow Cytometry & Diagnostics: Essential for developing fluorescent detection reagents and diagnostic assay kits requiring bright and stable signals.
• Biomolecular Tracking & Sensing: Enables real-time tracking of biomolecular interactions, localization, and dynamics in complex biological systems.
• Materials Science & Polymer Labeling: Applied in the synthesis of fluorescently tagged polymers, nanoparticles, and smart materials for research and quality control.
• High-Throughput Screening (HTS): Serves as a key component in developing fluorescent assays for drug discovery and biochemical analysis.

Basic Information

Product Name	Everfluor Fl, Se [4,4-Difluoro-5,7-Dimethyl-4-Bora-3A,4A-Diaza-S-Indacene-3-Propionic Acid, Succinimidyl Ester]
CAS No.	146616-66-2
Molecular Formula	C18H18BF2N3O5
Molecular Weight	405.16 g/mol
Synonyms	BODIPY® FL, SE; BODIPY FL-X, SE; 4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid, succinimidyl ester; BDP FL NHS ester; BODIPY 493/503 NHS ester; BODIPY-FL NHS; BODIPY-FL, SE, TFA of MP
EINECS	Contact for details

Quality Control

Our Everfluor Fl, SE is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and performance characteristics are provided with every shipment to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	~90,000 cm⁻¹M⁻¹ at ~503 nm
Fluorescence Emission λmax	~513 nm
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.