Description

Cy3 CAS NO 146397-20-8 is a high-purity cyanine dye derivative widely recognized for its strong fluorescence in the orange-red spectrum. This compound is critical for advanced biological research and diagnostic applications, offering excellent photostability and water solubility. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and biotechnology sectors for labeling, detection, and imaging.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, and nucleic acids (DNA, RNA) for detection and analysis.
• Bioimaging and Microscopy: Used as a vital stain in fluorescence microscopy (e.g., confocal, super-resolution) and in vivo imaging for cellular and subcellular structure visualization.
• Flow Cytometry: Employed as a fluorescent tag for cell sorting, immunophenotyping, and receptor studies.
• Diagnostic Assay Development: Key component in the formulation of fluorescent probes and kits for clinical diagnostics and high-throughput screening (HTS).
• Bioconjugation and Probe Synthesis: Serves as a reactive intermediate for synthesizing custom fluorescent probes and sensor molecules.
• Material Science Research: Applied in the development of fluorescent nanomaterials and polymer conjugates for sensing applications.

Basic Information

Product Name	Cy3
CAS No.	146397-20-8
Molecular Formula	C31H39KN2O6S2
Molecular Weight	639.88 g/mol
Synonyms	Cyanine3; Sulfo-Cyanine3; Cy™3; Cy3 NHS ester; 1H-Benz[e]indolium, 2-[2-[2-chloro-3-[(1,3-dihydro-1,3,3-trimethyl-2H-benz[e]indol-2-ylidene)ethylidene]-1-cyclopenten-1-yl]ethenyl]-1,3,3-trimethyl-, inner salt; 2-[2-[2-Chloro-3-[(1,3-dihydro-1,3,3-trimethyl-2H-benz[e]indol-2-ylidene)ethylidene]-1-cyclopenten-1-yl]ethenyl]-1,3,3-trimethyl-1H-benz[e]indolium chloride; Sulfo-Cy3
EINECS	Contact for details

Quality Control

Our Cy3 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry for absorbance/fluorescence validation. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with research-grade standards and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark green to purple solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Solubility	Soluble in water, DMSO, DMF
Moisture Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.