Description

Cy3 NHS Ester is a reactive, amine-reactive fluorescent labeling reagent widely used for the preparation of biological conjugates. This compound is critical for researchers and manufacturers who require high-sensitivity detection and visualization in complex biological systems. It is primarily utilized by professionals in the biotechnology, pharmaceutical, and diagnostic industries for applications such as fluorescence microscopy, flow cytometry, and immunoassays.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amines (lysine residues) for use in immunofluorescence (IF), Western blotting, and ELISA.
• Nucleic Acid Probe Synthesis: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Cell and Tissue Imaging: Preparation of labeled peptides, streptavidin, or other targeting molecules for high-resolution confocal and super-resolution microscopy.
• Flow Cytometry: Creation of antibody-dye conjugates for cell surface and intracellular marker detection.
• Drug Delivery & Biodistribution Studies: Tagging of nanoparticles, polymers, or therapeutic agents to track localization and uptake in vitro and in vivo.
• Biomolecular Interaction Analysis: Labeling of ligands or receptors for fluorescence polarization (FP) or other binding assays.

Basic Information

Product Name	Cy3 NHS Ester
CAS No.	146368-16-3
Molecular Formula	C37H41N3O6
Molecular Weight	623.75 g/mol
Synonyms	Cy3 N-Hydroxysuccinimide Ester; Sulfo-Cyanine3 NHS Ester; Cyanine3 NHS ester; 1-(3-Carboxypropyl)-1'-ethyl-3,3,3',3'-tetramethyl-5,5'-disulfonatoindocarbocyanine N-hydroxysuccinimide ester; Cy3® SE; NHS-Cy3; Cy3 Mono NHS Ester; 2-[(1E,3E)-5-[(1E,3E)-3-(1-Ethyl-3,3-dimethyl-5-sulfoindolin-2-ylidene)prop-1-en-1-yl]-3,3-dimethyl-1-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)indol-1-ium-2-yl]ethane-1-sulfonate
EINECS	Contact for details

Quality Control

Our Cy3 NHS Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) with detailed specifications are provided to guarantee the product meets the stringent requirements for life science research and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	548 - 552 nm
Emission (λmax)	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.