Description

Cy5 CAS NO 146368-15-2 is a near-infrared cyanine dye widely recognized for its high fluorescence quantum yield and excellent photostability. This compound is a critical tool for labeling and detection in advanced biomedical research and diagnostic applications. It is primarily utilized by researchers and manufacturers in the fields of life sciences, pharmaceutical development, and in vitro diagnostics for applications such as fluorescence microscopy, flow cytometry, and nucleic acid detection.

Application

• Fluorescence Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, and oligonucleotides for detection and imaging.
• Nucleic Acid Analysis: Labeling primers and probes for real-time PCR (qPCR), fluorescence in situ hybridization (FISH), and microarray assays.
• In Vivo and In Vitro Imaging: Used in preclinical small animal imaging and cell-based assays due to its deep tissue penetration in the near-infrared spectrum.
• Flow Cytometry & Microscopy: Enables multi-color analysis and high-resolution cellular and subcellular imaging.
• Diagnostic Assay Development: A key component in the development of lateral flow assays, ELISA, and other clinical diagnostic kits.
• Material Science Research: Incorporation into polymers and nanoparticles for the development of advanced sensing materials.

Basic Information

Product Name	Cy5
CAS No.	146368-15-2
Molecular Formula	C35H44N3O6S2+
Molecular Weight	666.87 g/mol (as cation)
Synonyms	Cyanine5; Sulfo-Cyanine5; Cy™5; Cy5 NHS ester; 1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,3,3-trimethyl-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-1,3,3-trimethyl-, perchlorate; 2-[5-(1,3-Dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-1,3-pentadienyl]-1,3,3-trimethyl-3H-indolium; Indodicarbocyanine 5
EINECS	Contact for details

Quality Control

Our Cy5 is produced under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for molar absorptivity verification, and functional testing in conjugation assays. Certificates of Analysis (COA) detailing all specifications are available upon request. We support compliance with relevant ISO and GMP guidelines for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Moisture Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.