Description

Cy5 Nhs Ester is a near-infrared fluorescent labeling reagent widely used for the covalent conjugation of biomolecules. This compound is critical for researchers and developers requiring high-sensitivity detection and imaging in complex biological systems. It is an essential tool for professionals in pharmaceutical development, proteomics, genomics, and in vitro diagnostics, enabling the creation of labeled antibodies, proteins, peptides, and oligonucleotides.

Application

• Antibody Labeling: Conjugation to monoclonal and polyclonal antibodies for fluorescence-activated cell sorting (FACS), immunofluorescence (IF), and Western blotting.
• Protein & Peptide Tagging: Site-specific labeling of proteins and peptides for biophysical studies, protein-protein interaction assays, and cellular imaging.
• Nucleic Acid Probes: Labeling of oligonucleotides, DNA, and RNA for fluorescence in situ hybridization (FISH), microarray analysis, and real-time PCR detection.
• Bioconjugation & Assay Development: Key reagent for developing high-throughput screening (HTS) assays, homogeneous assay formats, and diagnostic test kits.
• In Vivo & In Vitro Imaging: Preparation of targeted imaging probes for preclinical research in oncology, neurology, and inflammation studies.
• Material Science: Functionalization of nanoparticles, polymers, and surfaces for biosensing and diagnostic device applications.

Basic Information

Product Name	Cy5 Nhs Ester
CAS No.	146368-14-1
Molecular Formula	C37H45N3O6
Molecular Weight	627.78 g/mol
Synonyms	Cy5 NHS Ester; Sulfo-Cyanine5 NHS Ester; Cy5® N-Hydroxysuccinimide Ester; 1-(5-Carboxypentyl)-1'-propyl-3,3,3',3'-tetramethylindodicarbocyanine-5,5'-disulfonate NHS Ester; Cyanine5 NHS ester; N-Succinimidyl Cy5 ester; Cy5 fluorescent dye NHS ester; IR-786 NHS ester derivative
EINECS	Contact for details

Quality Control

Our Cy5 Nhs Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC purity analysis, identity confirmation by mass spectrometry (MS) and NMR, and functional performance validation. Certificates of Analysis (COA) detailing batch-specific results are provided to ensure traceability and compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Extinction Coefficient	≥ 250,000 L·mol⁻¹·cm⁻¹ (at ~649 nm)
Functional Performance	Conjugation efficiency ≥ 90% (vs. control)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.