Description

Cy3 CAS NO 146368-13-0 is a high-purity cyanine dye derivative, widely recognized as a critical fluorescent labeling reagent. Its primary value lies in its strong absorption in the visible spectrum and bright, stable fluorescence emission, enabling sensitive detection and quantification. This compound is essential for researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors who require reliable fluorescent probes for applications such as nucleic acid detection, protein labeling, and cellular imaging.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to oligonucleotides (DNA/RNA probes), antibodies, peptides, and other proteins for detection and analysis.
• Nucleic Acid Staining and Detection: Used in gel electrophoresis, Southern/Northern blotting, microarray analysis, and real-time PCR (qPCR) for sensitive visualization and quantification.
• Cellular and Tissue Imaging: Employed in fluorescence microscopy (including confocal and super-resolution) and flow cytometry for tracking cellular processes, receptor studies, and in vivo imaging.
• Diagnostic Assay Development: A key component in the formulation of fluorescent lateral flow assays, ELISA kits, and other in vitro diagnostic (IVD) reagents.
• Bioconjugation and Probe Synthesis: Serves as a reactive intermediate for synthesizing custom fluorescent probes and sensor molecules with NHS ester or other reactive groups.
• Material Science Research: Used in the development of fluorescent nanomaterials, sensors, and optoelectronic devices due to its photophysical properties.

Basic Information

Product Name	Cy3
CAS No.	146368-13-0
Molecular Formula	C31H31KN2O6S2
Molecular Weight	607.81 g/mol
Synonyms	Cyanine3; Sulfo-Cyanine3; Cy™3; Cy3 NHS ester; 1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,3,3-trimethyl-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-, inner salt, 4-methylbenzenesulfonate; 2-[5-(1,3-Dihydro-1,3,3-trimethyl-2H-benz[e]indol-2-ylidene)-1,3-pentadienyl]-1-ethyl-3,3-dimethyl-1H-benz[e]indolium 4-methylbenzenesulfonate; Sulfo-Cy3; Cy3 fluorescent dye
EINECS	Contact for details

Quality Control

Our Cy3 is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and functional testing in relevant assay formats. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance specifications is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. For long-term storage, keep the product under an inert atmosphere (e.g., argon or nitrogen) to prevent degradation. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark green to purple solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Fluorescence Quantum Yield	Contact for details
Solubility	Soluble in DMSO, DMF, water (sulfonated forms)
Moisture Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.