Description

Cy5 CAS NO 146368-11-8 is a near-infrared cyanine dye belonging to the indocarbocyanine family, widely recognized for its strong fluorescence and excellent photostability. This compound is a critical tool for labeling and detection in advanced life science research and diagnostic applications. It is essential for researchers and manufacturers in the fields of molecular biology, medical diagnostics, and pharmaceutical development who require high-performance fluorescent probes.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, and oligonucleotides for detection and quantification.
• Nucleic Acid Analysis: Labeling primers and probes for real-time PCR (qPCR), fluorescence in situ hybridization (FISH), and microarray applications.
• In Vivo and In Vitro Imaging: Used in small animal imaging and cell-based assays due to its deep tissue penetration and minimal autofluorescence interference.
• Flow Cytometry: As a fluorescent tag for cell surface and intracellular marker analysis.
• Diagnostic Assay Development: A key component in lateral flow assays, ELISA, and other immunoassay platforms.
• Material Science: Incorporation into polymers and nanoparticles for creating functional fluorescent materials and sensors.

Basic Information

Product Name	Cy5
CAS No.	146368-11-8
Molecular Formula	C35H44N2O6S2+
Molecular Weight	644.86 g/mol (cation)
Synonyms	Cyanine5; Sulfo-Cyanine5; Cy™5; Cy5 NHS Ester; 1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,3,3-trimethyl-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-1,3,3-trimethyl-, perchlorate; 2-[5-(1,3-Dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-1,3-pentadienyl]-1,3,3-trimethyl-3H-indolium; IRIS 5
EINECS	Contact for details

Quality Control

Our Cy5 is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance and fluorescence verification, and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, store at 2-8°C. Allow the vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.