Description

Cyanine 5 Bisacid [Equivalent To Cy5 Bisacid] CAS NO 146368-10-7 is a high-purity, water-soluble, near-infrared fluorescent dye intermediate essential for advanced bioconjugation. Its core value lies in providing a reactive carboxylic acid handle for stable amide bond formation, enabling the labeling of biomolecules like proteins, peptides, and antibodies with minimal interference to their biological activity. This compound is critically needed by researchers and manufacturers in the biotechnology, pharmaceutical diagnostics, and life science research sectors for developing sensitive assays, in vivo imaging probes, and diagnostic kits.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for detection and imaging.
• In Vivo and In Vitro Imaging: Synthesis of near-infrared (NIR) probes for deep-tissue fluorescence imaging in preclinical research.
• Diagnostic Assay Development: Key component in manufacturing fluorescent reagents for ELISA, flow cytometry, and immunohistochemistry.
• Drug Discovery & Development: Used to label target molecules for high-throughput screening (HTS) and pharmacokinetic studies.
• Biomaterial and Nanoparticle Functionalization: Tagging polymers, dendrimers, and nanoparticles for targeted delivery and tracking.
• DNA/RNA Labeling: Preparation of fluorescently tagged nucleotides for sequencing and microarray applications.

Basic Information

Item	Details
Product Name	Cyanine 5 Bisacid [Equivalent To Cy5 Bisacid]
CAS No.	146368-10-7
Molecular Formula	C37H45N2O4+
Molecular Weight	581.77 g/mol (for cation)
Synonyms	Cy5 Bisacid; Cy5 dicarboxylic acid; 1,1'-(4,4,7,7-Tetramethyl-4,7-dihydro-5H-4,7-methano-2H-benzimidazol-2-ylidene)bis[but-3-en-1-ylidene]bis[1-ethyl-3,3-dimethyl-3H-indolium] dichloride; Sulfo-Cyanine5 bisacid; NIR dye bisacid; Fluorescent cyanine dye bis-acid
EINECS	Contact for details

Quality Control

Our Cyanine 5 Bisacid is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) providing purity, absorbance/fluorescence data, and impurity profiles is supplied with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); keep the container tightly sealed in a desiccated environment to maintain stability and prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.