Description

5,6-Carboxyfluorescein Dipivalate is a chemically modified, protected derivative of fluorescein, designed for enhanced stability and controlled reactivity. This compound is a critical intermediate and labeling reagent in the synthesis of advanced fluorescent probes and conjugates. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for applications requiring high-sensitivity detection and imaging.

Application

• Fluorescent Probe Synthesis: A key protected intermediate for preparing active esters (e.g., NHS esters, SE) used to label proteins, antibodies, peptides, and nucleic acids.
• Cell Biology & Imaging: Used in the development of intracellular pH indicators and tracers for studying cell viability, membrane permeability, and gap junction communication.
• Diagnostic Assay Development: Employed in the manufacture of reagents for ELISA, fluorescence immunoassays (FIA), and lateral flow tests.
• Drug Delivery Research: Acts as a fluorescent tag for tracking the biodistribution, cellular uptake, and release kinetics of nanoparticle and liposomal drug carriers.
• Flow Cytometry: Serves as a precursor for fluorescent dyes used in cell sorting and analysis.
• Bioconjugation Chemistry: The dipivalate (pivaloyl) protecting groups allow for selective deprotection and conjugation under mild conditions, enabling precise labeling strategies.

Basic Information

Product Name	5,6-Carboxyfluorescein Dipivalate
CAS No.	144676-12-0
Molecular Formula	C33H32O10
Molecular Weight	588.60 g/mol
Synonyms	5(6)-Carboxyfluorescein Di(pivaloyloxymethyl) Ester; 5,6-FAM Dipivalate; 5(6)-Carboxyfluorescein Dipivaloyloxymethyl Ester; CFDA Dipivalate; 5,6-CFDP; Fluorescein-5(6)-carboxylic acid dipivaloyloxymethyl ester; 2-[3,6-Bis[(2,2-dimethylpropanoyloxy)methoxy]-9H-xanthen-9-yl]benzoic acid
EINECS	Contact for details

Quality Control

Our 5,6-Carboxyfluorescein Dipivalate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and bioconjugation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.