Description

n-(4-(4-Azidobenzamido)Butyl)-3-(2'-Pyridyldithio)Propionamide is a heterobifunctional crosslinking reagent designed for advanced bioconjugation and protein modification workflows. This compound matters for its dual functionality, combining a photoactivatable aryl azide group with a cleavable pyridyldithio moiety, enabling controlled, sequential conjugation strategies. It is essential for researchers and manufacturers in biopharmaceutical development, proteomics, and diagnostic assay kit production who require precise, site-specific labeling of biomolecules.

Application

• Bioconjugation and Protein Labeling: Site-specific crosslinking of proteins, antibodies, or peptides to other biomolecules, surfaces, or solid supports.
• Affinity Chromatography Ligand Immobilization: Covalent attachment of antibodies or other capture agents to resin matrices for purification columns.
• Diagnostic Assay Development: Conjugation of detection enzymes or fluorescent probes to antibodies for ELISA, lateral flow, or other immunoassays.
• ADC (Antibody-Drug Conjugate) Linker Exploration: Used in research for constructing cleavable linkers between monoclonal antibodies and cytotoxic payloads.
• Surface Functionalization: Modification of gold nanoparticles, biosensor chips, or other materials with biomolecules via the dithiol group.
• Proteomics and Protein Interaction Studies: Photo-crosslinking applications to capture transient protein-protein interactions.

Basic Information

Product Name	n-(4-(4-Azidobenzamido)Butyl)-3-(2'-Pyridyldithio)Propionamide
CAS No.	143706-33-6
Molecular Formula	C19H21N7O2S2
Molecular Weight	443.55 g/mol
Synonyms	4-Azido-2,3,5,6-tetrafluorobenzoic acid succinimidyl ester; Heterobifunctional crosslinker, Azide-PDPH; N-[4-(4-Azidobenzamido)butyl]-3-(2-pyridyldithio)propionamide; PDPH-Azide; 3-(2-Pyridyldithio)-N-[4-(4-azidobenzamido)butyl]propionamide; Azido-PDPH; Photo-cleavable bioconjugation reagent; Aryl azide PDPH crosslinker
EINECS	Contact for details

Quality Control

Our n-(4-(4-Azidobenzamido)Butyl)-3-(2'-Pyridyldithio)Propionamide is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and development. Each lot undergoes comprehensive analytical characterization, including HPLC purity analysis, NMR structural confirmation, and functional group verification. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, identity, and suitability for bioconjugation applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.