Description

5-(Aminomethyl)Fluorescein Hydrochloride is a chemically reactive derivative of fluorescein, widely used as a versatile labeling reagent for biomolecules. This compound is essential for researchers and manufacturers requiring a bright, stable fluorescent tag with an amine-reactive functional group. It is primarily utilized in the life sciences, diagnostics, and pharmaceutical development sectors for applications such as protein conjugation, immunoassay development, and fluorescence microscopy.

Application

• Bioconjugation & Protein Labeling: Acts as a primary amine-reactive probe for covalently labeling antibodies, peptides, and other proteins for fluorescence-based assays.
• Immunoassay Development: Used to prepare fluorescently-labeled detection antibodies or antigens in ELISA, lateral flow assays, and other diagnostic platforms.
• Fluorescence Microscopy & Imaging: Serves as a bright, photostable fluorescent tag for visualizing cellular structures and tracking biomolecules in live or fixed cells.
• Flow Cytometry: Employed to create fluorescent conjugates for cell surface marker detection and analysis.
• Biochemical Research: A key tool for studying protein-protein interactions, receptor-ligand binding, and enzymatic activity through fluorescence detection.
• Diagnostic Reagent Manufacturing: Integrated into the production of high-sensitivity diagnostic kits and biosensors.

Basic Information

Product Name	5-(Aminomethyl)Fluorescein Hydrochloride
CAS No.	141749-41-9
Molecular Formula	C22H17NO5 • HCl
Molecular Weight	419.83 g/mol (Free base: 383.38 g/mol)
Synonyms	5-(Aminomethyl)fluorescein HCl; 5-(Aminomethyl)fluorescein hydrochloride; Fluoresceinamine isomer I hydrochloride; 6-Aminofluorescein hydrochloride (common name for isomer mixture); 5-(Aminomethyl)-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one hydrochloride; FAM-amine hydrochloride; Aminomethylfluorescein HCl
EINECS	Contact for details

Quality Control

Our 5-(Aminomethyl)Fluorescein Hydrochloride is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; desiccants are recommended for long-term storage. Keep container tightly sealed when not in use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~492 nm
Emission Maximum	~518 nm
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.