Description

4-(Trifluoromethyl)Umbelliferyl Butyrate is a specialized fluorogenic ester derivative of umbelliferone, designed for high-sensitivity enzymatic assays. This compound matters as a critical substrate for detecting and quantifying lipase and esterase activity, offering superior sensitivity and a stable fluorescent signal upon enzymatic cleavage. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for enzyme kinetics studies and high-throughput screening applications.

Application

• Lipase & Esterase Activity Assays: A key fluorogenic substrate for the detection and quantification of lipase and esterase enzymes in biochemical research.
• High-Throughput Screening (HTS): Used in pharmaceutical discovery for screening compound libraries against enzymatic targets in microplate formats.
• Diagnostic Reagent Development: Serves as a core component in the formulation of diagnostic kits for pancreatic function and metabolic disorders.
• Enzyme Kinetics Studies: Enables precise measurement of kinetic parameters (Km, Vmax) for characterizing novel enzymes or inhibitors.
• Biotechnology Process Monitoring: Applied in fermentation and biocatalysis processes to monitor enzyme production and activity in real-time.
• Food Science & Quality Control: Utilized to assay lipase activity in food products, ingredients, and during enzymatic food processing.

Basic Information

Product Name	4-(Trifluoromethyl)Umbelliferyl Butyrate
CAS No.	141573-64-0
Molecular Formula	C15H13F3O4
Molecular Weight	314.26 g/mol
Synonyms	4-Trifluoromethylumbelliferyl butyrate; 4-Methyl-7-trifluoromethylcoumarin butyrate; 4-Methyl-7-(trifluoromethyl)-2H-1-benzopyran-2-one butanoate; 7-Trifluoromethyl-4-methylumbelliferyl butyrate; TFU-B; 4-MU-TFM butyrate; Umbelliferyl 4-(trifluoromethyl) butyrate; 4-(Trifluoromethyl)-7-hydroxycoumarin butyrate ester
EINECS	Contact for details

Quality Control

Our 4-(Trifluoromethyl)Umbelliferyl Butyrate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%
Fluorogenic Activity	Functional in standard lipase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.