Description

4-Azido-2-Nitrophenyl Polyethylene Glycol is a specialized heterobifunctional PEG derivative designed for advanced bioconjugation and surface modification. This compound matters for its unique combination of a photoactivatable azido group and a nitrophenyl moiety, enabling controlled, light-induced crosslinking and labeling under mild conditions. It is essential for researchers and manufacturers in the fields of biopharmaceutical development, advanced material science, and diagnostic assay creation who require precise and stable attachment of biomolecules.

Application

• Bioconjugation Reagent: For the site-specific crosslinking of proteins, peptides, antibodies, and oligonucleotides via photoactivation.
• Surface Functionalization: To create bio-inert or bioactive coatings on nanoparticles, quantum dots, microarrays, and medical device surfaces.
• Hydrogel Formation: As a key crosslinker in the development of photopolymerizable hydrogels for 3D cell culture and tissue engineering scaffolds.
• Drug Delivery Systems: For constructing stimuli-responsive drug carriers and antibody-drug conjugates (ADCs) with controlled release profiles.
• Diagnostic Assays: In the development of sensitive immunoassays and biosensors by immobilizing capture molecules on solid supports.
• Polymer Chemistry: As a macro-initiator or functional chain-end for synthesizing block copolymers with tailored properties.

Basic Information

Product Name	4-Azido-2-Nitrophenyl Polyethylene Glycol
CAS No.	140842-24-6
Molecular Formula	Typically CnH2n+1OmN3 (PEG chain length variable)
Molecular Weight	Variable (dependent on PEG chain length; e.g., 1kDa, 3.4kDa, 5kDa, 10kDa)
Synonyms	ANP-PEG; 2-Nitro-4-azidophenyl Polyethylene Glycol; 4-Azido-2-nitro-PEG; PEG-ANP; Photoactivatable PEG; Heterobifunctional PEG Azide; Nitrophenyl Azido PEG; Light-activatable Crosslinker
EINECS	Contact for details

Quality Control

Our 4-Azido-2-Nitrophenyl Polyethylene Glycol is manufactured under strict quality management systems. Each batch is characterized and tested to ensure high purity, consistent molecular weight, and functional group integrity. Comprehensive Certificates of Analysis (COA) are provided, detailing results from NMR (¹H and ¹³C), HPLC, and functional group analysis. We support cGMP manufacturing for pharmaceutical applications and can provide material suitable for use under ISO 13485 standards for medical devices.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation and degradation. For long-term stability, desiccate to minimize moisture uptake. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous liquid
PEG Average Molecular Weight (Mn)	As ordered (e.g., 1,000 Da, 3,400 Da, 5,000 Da)
PEG Dispersity (Đ)	≤ 1.05
Assay (Functional Group)	≥ 95% (by ¹H NMR)
Purity (HPLC)	≥ 98%
Identification (IR)	Conforms to structure (Azido, Nitro, PEG peaks)
Water Content (KF)	≤ 1.0%
Residual Solvent (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.