Description

n-Hydroxysuccinimidyl Acetoacetate is a highly reactive, bifunctional ester widely used as a versatile linker and cross-linking agent in bioconjugation chemistry. Its core value lies in enabling the stable attachment of acetoacetyl groups to primary amines, facilitating the development of advanced materials and bioprobes. This compound is essential for researchers and manufacturers in the pharmaceutical, diagnostics, and life science sectors who require precise and efficient protein modification, polymer functionalization, and surface immobilization strategies.

Application

• Protein and Antibody Labeling: Conjugation of fluorescent dyes, drugs, or other payloads to biomolecules for diagnostics and targeted therapies.
• Polymer Functionalization: Introduction of reactive ketone groups into polymers or hydrogels for subsequent cross-linking or ligand attachment.
• Surface Chemistry: Modification of sensor chips, microarrays, and nanoparticles to create bioactive surfaces for assays and separations.
• Peptide Synthesis: Used as an activated ester for the solid-phase synthesis of peptides containing acetoacetylated amino acids.
• Biomaterial Development: Fabrication of cross-linked biomaterials with tunable properties for tissue engineering and drug delivery systems.
• Affinity Chromatography Ligand Synthesis: Immobilization of small molecules or peptides onto resin supports.

Basic Information

Product Name	n-Hydroxysuccinimidyl Acetoacetate
CAS No.	139549-71-6
Molecular Formula	C8H9NO5
Molecular Weight	199.16 g/mol
Synonyms	Acetoacetic Acid N-Hydroxysuccinimide Ester; NHS-Acetoacetate; Succinimidyl Acetoacetate; 3-(2,5-Dioxopyrrolidin-1-yl) 3-oxobutanoate; 2,5-Dioxopyrrolidin-1-yl 3-oxobutanoate; AcAc-OSu; N-Hydroxysuccinimide Acetoacetate Ester; AA-NHS
EINECS	Contact for details

Quality Control

Our n-Hydroxysuccinimidyl Acetoacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and IR spectroscopy, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	95.0 - 105.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.