Description

6-(Aminomethyl)Fluorescein is a chemically reactive derivative of fluorescein, featuring a primary amine group that enables facile conjugation to various biomolecules and surfaces. This compound is essential for creating highly sensitive fluorescent probes and labels used in advanced diagnostic and research applications. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, assay development, and diagnostic kit production, where reliable and bright fluorescent signaling is required.

Application

• Fluorescent Labeling Agent: For covalent conjugation to proteins, antibodies, peptides, and oligonucleotides to create fluorescent probes.
• Bioconjugation Chemistry: Acts as a key intermediate in the synthesis of custom fluorescent dyes and tags via amine-reactive chemistry (e.g., NHS ester formation).
• Diagnostic Assay Development: Used in the manufacturing of fluorescent immunoassays (FLIA), lateral flow tests, and other point-of-care diagnostic devices.
• Cell and Tissue Staining: Employed in the preparation of fluorescent stains for microscopy and flow cytometry applications.
• Enzyme Substrate Synthesis: Serves as a precursor for developing fluorogenic substrates to detect enzymatic activity (e.g., protease or phosphatase assays).
• Sensor and Material Science: Incorporated into polymers, nanoparticles, and biosensors to impart fluorescence for detection and imaging purposes.

Basic Information

Product Name	6-(Aminomethyl)Fluorescein
CAS No.	138588-54-2
Molecular Formula	C22H17NO5
Molecular Weight	375.37 g/mol
Synonyms	6-(Aminomethyl)fluorescein; 5(6)-Aminomethylfluorescein; Fluorescein-6-methylamine; 2-(6-Aminomethyl-3-oxo-3H-xanthen-9-yl)benzoic acid; 6-AMF; Aminomethylfluorescein; 6-Aminomethyl-3H-xanthen-3-one 9-(2-carboxyphenyl) derivative
EINECS	Contact for details

Quality Control

Our 6-(Aminomethyl)Fluorescein is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to air and humidity.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.