Description

5-(Aminomethyl)Fluorescein is a chemically reactive derivative of fluorescein, featuring a primary amine group that enables facile conjugation to various biomolecules and surfaces. This compound is crucial for creating highly sensitive fluorescent probes and labels used in advanced diagnostic and research applications. It is primarily needed by biotechnology, pharmaceutical, and academic research sectors for applications in bioimaging, immunoassays, and molecular diagnostics.

Application

• Fluorescent Labeling Agent: Conjugation to antibodies, proteins, peptides, and oligonucleotides for use in fluorescence microscopy (e.g., confocal, super-resolution) and flow cytometry.
• Biosensor Development: Key component in the synthesis of enzyme substrates and activity-based probes for detecting protease, phosphatase, and other enzymatic activities.
• Diagnostic Assays: Used in the development of fluorescent immunoassays (FLISA), lateral flow tests, and in-situ hybridization (FISH) probes.
• Cell Biology Research: Labeling of cellular components for tracking intracellular trafficking, membrane dynamics, and receptor-ligand interactions.
• Material Science: Functionalization of nanoparticles, polymers, and surfaces to create fluorescent materials for sensing and imaging applications.
• Drug Discovery: High-throughput screening (HTS) assays to monitor biochemical interactions and cellular responses.

Basic Information

Product Name	5-(Aminomethyl)Fluorescein
CAS No.	138588-53-1
Molecular Formula	C22H17NO5
Molecular Weight	375.38 g/mol
Synonyms	5-(Aminomethyl)fluorescein; 5-(Aminomethyl)-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; Fluoresceinamine isomer I; 5-Fluoresceinamine; 5-Aminomethylfluorescein; 5-AMF; FAM-amine; 5-(Aminomethyl)-3',6'-dihydroxyfluoran
EINECS	Contact for details

Quality Control

Our 5-(Aminomethyl)Fluorescein is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional testing for labeling efficiency. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorescence Properties	Ex/Em ~492/518 nm (pH >8)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.