Description

Calcium Green CAS NO 138067-55-7 is a high-purity, cell-permeant fluorescent calcium indicator widely used in biological research. This compound is essential for the quantitative measurement of intracellular calcium ion (Ca²⁺) concentrations in live cells, providing critical insights into cellular signaling and physiological processes. It is a vital tool for researchers and professionals in the fields of neuroscience, pharmacology, cell biology, and drug discovery, where precise calcium imaging is required.

Application

• Live-Cell Calcium Imaging: Real-time visualization and quantification of intracellular calcium flux in neurons, cardiomyocytes, and other cell types.
• Neuroscience Research: Studying calcium signaling in synaptic transmission, neuronal excitability, and neurodegenerative disease models.
• Drug Screening & Pharmacology: High-throughput screening (HTS) assays to evaluate the effect of pharmaceutical compounds on calcium channel activity and cellular response.
• Cardiovascular Research: Monitoring calcium transients in cardiac muscle cells to understand contractility and arrhythmia mechanisms.
• Cell Physiology Studies: Investigating calcium's role in apoptosis, muscle contraction, secretion, and gene expression.
• Fluorescence Microscopy & Flow Cytometry: Compatible with standard FITC filter sets for versatile detection in imaging and cell sorting applications.

Basic Information

Product Name	Calcium Green
CAS No.	138067-55-7
Molecular Formula	C₄₆H₄₈K₂N₄O₂₃
Molecular Weight	1130.19 g/mol
Synonyms	Calcium Green-1; Calcium Green 1; Calcium Green-1, Pentapotassium Salt; Calcium Green™ 1; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-[2-amino-5-methylphenoxy]ethane-N,N,N',N'-tetraacetic Acid, Pentapotassium Salt; BAPTA-based Ca²⁺ indicator; Fluorescent calcium indicator
EINECS	Contact for details

Quality Control

Our Calcium Green is manufactured under strict quality protocols to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and functional validation in cell-based assays. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming compliance with our stringent specifications for biological use.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorbance (λmax)	~506 nm
Emission (λmax)	~531 nm
Calcium Binding Affinity (Kd)	~190 nM (typical, varies with conditions)
Water Content (KF)	≤ 5.0%
Functional Activity	Validated in cell-based calcium imaging

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.