Description

Bodipy-Verapamil CAS NO 137759-83-2 is a fluorescently labeled derivative of the calcium channel blocker verapamil, designed for advanced research applications. This compound is critical for studying drug transport mechanisms, particularly P-glycoprotein (P-gp) mediated efflux and multidrug resistance (MDR) in cancer cells. It is an essential tool for pharmaceutical researchers, cell biologists, and toxicologists working in drug discovery and development. The fluorescent Bodipy tag allows for real-time visualization and quantification of cellular uptake and efflux processes.

Application

• Multidrug Resistance (MDR) Research: Used as a fluorescent substrate to study the function and inhibition of P-glycoprotein and other ABC transporters in cancer cell lines.
• Cellular Efflux Assays: Enables high-throughput screening (HTS) and flow cytometry-based assays to identify potential P-gp inhibitors or modulators.
• Drug Transport & Permeability Studies: Applied in models of the blood-brain barrier (BBB) and intestinal epithelium to assess drug permeability and active efflux.
• Live-Cell Imaging: Facilitates real-time, confocal microscopy visualization of drug accumulation and localization within living cells.
• Pharmacokinetics & Toxicology: Serves as a probe to investigate drug-drug interactions that affect transport pathways in preclinical models.
• Biochemical Research: Used in the development and validation of in vitro assays for transporter protein activity and binding studies.

Basic Information

Product Name	Bodipy-Verapamil
CAS No.	137759-83-2
Molecular Formula	C48H58BF2N3O4
Molecular Weight	777.80 g/mol
Synonyms	BODIPY® FL Verapamil; BODIPY-FL-Verapamil; BODIPY FL Verapamil Hydrochloride; BODIPY®-Verapamil; Green Fluorescent Verapamil Analog; 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionyl Verapamil; Fluorescent Verapamil; P-gp Fluorescent Substrate.
EINECS	Contact for details

Quality Control

Our Bodipy-Verapamil is produced under strict quality management protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards required for fluorescence-based assays. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, spectral properties, and performance data.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. For long-term storage, solutions should be prepared in anhydrous DMSO and aliquoted to avoid repeated freeze-thaw cycles. Allow the product to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Excitation/Emission Maxima	~503 nm / ~512 nm
Extinction Coefficient	~90,000 cm⁻¹M⁻¹
Solubility	Soluble in DMSO, DMF, ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.