Description

5-Azido-2'-Deoxyuridine is a modified nucleoside analog of significant interest in biochemical and pharmaceutical research. This compound serves as a versatile chemical probe and building block, primarily valued for its ability to undergo bioorthogonal click chemistry reactions via its azido functional group. It is essential for researchers and developers in the fields of medicinal chemistry, nucleic acid research, and molecular biology who require high-purity, specialized nucleosides for labeling, cross-linking, and prodrug development.

Application

• Bioconjugation & Labeling: Acts as a key reagent for site-specific labeling of nucleic acids and proteins via copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry.
• Antiviral Research: Investigated as a potential prodrug or active metabolite in the development of novel antiviral agents, particularly against herpesviruses.
• DNA/RNA Cross-linking Studies: Used to study nucleic acid structure and protein-nucleic acid interactions through photoaffinity or chemical cross-linking.
• Metabolic Pathway Tracing: Employed as a metabolic tracer to study the incorporation and turnover of nucleosides in cellular systems.
• Medicinal Chemistry Synthesis: Serves as a critical synthetic intermediate for the preparation of more complex nucleoside-based drug candidates and molecular probes.
• Biomaterial Functionalization: Utilized to functionalize surfaces and polymers with bioactive nucleoside motifs for tissue engineering and biosensor applications.

Basic Information

Product Name	5-Azido-2'-Deoxyuridine
CAS No.	137359-50-3
Molecular Formula	C9H11N5O5
Molecular Weight	269.22 g/mol
Synonyms	5-Azido-2'-deoxyuridine; 5-Azidodeoxyuridine; 5-N3-dU; Azidothymidine analog; 1-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-azidopyrimidine-2,4(1H,3H)-dione; 5-Azido-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
EINECS	Contact for details

Quality Control

Our 5-Azido-2'-Deoxyuridine is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles, typically determined by HPLC, NMR, and mass spectrometry. We adhere to stringent quality management protocols to meet the exacting standards of the pharmaceutical and biotechnology research sectors.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label or COA. This compound is strictly light-sensitive (protect from all light exposure) and may be moisture-sensitive; therefore, containers should be kept sealed in a cool, dry place. For long-term storage, consider storing desiccated under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.