Description

5-Chloromethylfluorescein Diacetate is a protected, cell-permeable derivative of fluorescein, serving as a critical intermediate in the synthesis of advanced fluorescent probes and labels. This compound matters for its role in creating highly specific conjugates used in bioimaging, diagnostics, and cellular research, where precise targeting and signal generation are paramount. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic reagent industries for applications in fluorescence microscopy, flow cytometry, and assay development.

Application

• Fluorescent Probe Synthesis: A key precursor for creating custom fluorescent labels and tags for biomolecules (e.g., proteins, antibodies, nucleic acids).
• Live-Cell Imaging: Used to develop cell-permeable fluorescent indicators for tracking intracellular processes and localization in real-time.
• Diagnostic Assays: Employed in the manufacture of reagents for enzyme activity assays, immunoassays, and other clinical diagnostic tests.
• Flow Cytometry: Serves as a building block for fluorescent dyes used in cell sorting and analysis.
• Bioconjugation Chemistry: The chloromethyl group provides a reactive handle for covalent attachment to various target molecules under controlled conditions.
• Research & Development: A valuable tool in academic and industrial R&D for developing new optical imaging techniques and sensing platforms.

Basic Information

Product Name	5-Chloromethylfluorescein Diacetate
CAS No.	136832-63-8
Molecular Formula	C27H19ClO7
Molecular Weight	490.89 g/mol
Synonyms	5-Chloromethylfluorescein Diacetate; CMFDA Diacetate; 5-(Chloromethyl)fluorescein Diacetate; Chloromethylfluorescein Diacetate; 6-Carboxyfluorescein Diacetate (5-Chloromethyl) Derivative; CellTracker™ Green CMFDA Diacetate (related trade name); Fluorescein Diacetate, 5-Chloromethyl Derivative; 2',7'-Bis(acetyloxy)-5-(chloromethyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5'-carboxylic Acid
EINECS	Contact for details

Quality Control

Our 5-Chloromethylfluorescein Diacetate is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive research and manufacturing applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is light-sensitive (store away from light). For long-term storage, consider under inert atmosphere to maximize stability.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.