Description

6-Iodoacetamidotetramethylrhodamine is a reactive, thiol-specific fluorescent labeling reagent derived from the classic rhodamine dye family. This compound is critical for researchers requiring high-sensitivity, site-specific conjugation of fluorescent probes to proteins, peptides, and other biomolecules containing cysteine residues. It is primarily needed by scientists and manufacturers in the fields of bioconjugation, proteomics, and advanced diagnostics for creating labeled antibodies, imaging agents, and assay components.

Application

• Bioconjugation and Protein Labeling: Site-specific fluorescent labeling of cysteine residues in antibodies, enzymes, and other proteins for imaging and detection applications.
• Proteomics Research: Used as a thiol-reactive tag in activity-based protein profiling (ABPP) and other chemical proteomics strategies to study enzyme function and discovery.
• Fluorescent Probe Synthesis: A key building block for creating custom fluorescent sensors, molecular beacons, and imaging agents with red-emitting properties.
• Diagnostic Assay Development: Conjugation to detection molecules in lateral flow assays (LFAs), ELISA, and other immunoassay platforms to generate signal.
• Cell and Tissue Imaging: Preparation of labeled ligands or tracers for fluorescence microscopy (e.g., confocal, super-resolution) and flow cytometry.
• Pharmaceutical Analysis: Derivatization agent for the HPLC or LC-MS analysis of thiol-containing drugs and metabolites.

Basic Information

Product Name	6-Iodoacetamidotetramethylrhodamine
CAS No.	136538-85-7
Molecular Formula	C25H22IN3O4
Molecular Weight	579.37 g/mol
Synonyms	6-Iodoacetamido TAMRA; 6-IATR; 5(6)-Iodoacetamidotetramethylrhodamine; TAMRA iodoacetamide; Rhodamine 6G iodoacetamide; 6-TAMRA iodoacetamide; 6-Carboxytetramethylrhodamine iodoacetamide; N-(5-Carboxypentyl)-4-((5-(iodoacetamido)pentyl)amino)-9-(dimethylamino)-3H-xanthen-3-ylidene)-N,N-dimethylbenzenaminium
EINECS	Contact for details

Quality Control

Our 6-Iodoacetamidotetramethylrhodamine is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the needs of research and diagnostic-grade chemical synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M]+
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Functional Performance	Conjugates efficiently with standard thiol substrates

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.