Description

5-(Aminoacetamido)Fluorescein (Fluoresceinylglycineamide) is a chemically reactive derivative of fluorescein, designed for covalent conjugation to biomolecules. This compound matters because it serves as a critical fluorescent labeling reagent, enabling the sensitive detection and quantification of proteins, peptides, and other biological targets. Researchers and manufacturers in the fields of bioconjugation, immunoassay development, and diagnostic kit production rely on its bright fluorescence and amine-reactive properties for creating high-performance detection systems.

Application

• Fluorescent Labeling of Antibodies and Proteins: Conjugation via its amino-reactive group for use in fluorescence microscopy (FL), flow cytometry, and Western blotting.
• Immunoassay Development: Key reagent for developing fluorescent enzyme-linked immunosorbent assays (FELISA) and other diagnostic assays.
• Bioconjugation & Probe Synthesis: Synthesis of fluorescent probes and tracers for cellular imaging and in vivo studies.
• Diagnostic Kit Manufacturing: Used in the production of commercial diagnostic kits for clinical and research laboratories.
• Biomolecule Detection & Quantification: Enables sensitive detection of low-abundance targets in complex biological matrices.
• High-Throughput Screening (HTS): Fluorescent tag in assay development for drug discovery and biochemical analysis.

Basic Information

Product Name	5-(Aminoacetamido)Fluorescein (Fluoresceinylglycineamide)
CAS No.	136091-82-2
Molecular Formula	C25H18N2O7
Molecular Weight	458.42 g/mol
Synonyms	Fluoresceinylglycineamide; 5-(Glycinamido)fluorescein; Fluorescein-5-glycinamide; 5-(2-Aminoacetamido)fluorescein; 5-[(Aminoacetyl)amino]fluorescein; Fluorescein isothiocyanate isomer I precursor; Reactive Fluorescein derivative
EINECS	Contact for details

Quality Control

Our 5-(Aminoacetamido)Fluorescein is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification, and functional performance validation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95%
Absorption Max (λmax)	~490 nm
Emission Max	~515 nm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Moisture Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.