Description

2,3-Bis(3-Chlorophenyl)-5-Phenyltetrazolium Chloride is a specialized tetrazolium salt derivative widely used as a vital reagent in biochemical and analytical applications. Its core value lies in its role as a redox indicator and electron acceptor, particularly in assays for cell viability and dehydrogenase activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors.

Application

• Cell Viability and Cytotoxicity Assays: Used as a key substrate in MTT and related assays to measure mitochondrial activity in living cells.
• Microbiological Testing: Employed as an indicator for microbial growth and metabolic activity in environmental and clinical samples.
• Enzyme Activity Studies: Serves as an electron acceptor in assays for various dehydrogenase enzymes.
• Pharmaceutical Research: Utilized in high-throughput screening (HTS) to evaluate the effects of drug candidates on cell proliferation.
• Biochemical Reagent: Acts as a sensitive chromogenic agent in diagnostic kits and research protocols.
• Material Science Research: Applied in studies involving redox-active polymers and coatings.

Basic Information

Product Name	2,3-Bis(3-Chlorophenyl)-5-Phenyltetrazolium Chloride
CAS No.	135788-07-7
Molecular Formula	C20H13Cl3N4
Molecular Weight	415.70 g/mol
Synonyms	3,3'-(1H-Tetrazole-2,5-diyl)bis(1-chlorobenzene) Chloride; 2,3-Bis(3-chlorophenyl)-5-phenyl-2H-tetrazolium Chloride; 2,3-Bis(m-chlorophenyl)-5-phenyltetrazolium Chloride; m-Cl-PTZ; Tetrazolium Chloride Derivative; TTC Derivative; Cell Viability Assay Reagent; MTT Analog
EINECS	Contact for details

Quality Control

Our 2,3-Bis(3-Chlorophenyl)-5-Phenyltetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. Keep the container tightly sealed when not in use.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.