Description

Fluorescein Di-p-Guanidinobenzoate Hydrochloride is a chemically modified fluorescein derivative designed for high-sensitivity detection and quantification applications. This compound is valued for its ability to act as a highly specific and sensitive substrate or probe, leveraging the strong fluorescence of the fluorescein core. It is primarily utilized by researchers and manufacturers in the fields of biochemical diagnostics, protease activity assays, and advanced material science. The product is supplied as a high-purity reagent to ensure reliable and reproducible experimental and production outcomes.

Application

• Protease and Enzyme Activity Assays: Serves as a fluorogenic substrate for the detection and kinetic study of specific proteolytic enzymes, including trypsin and related serine proteases.
• Biochemical Research Reagent: Used in life science research for developing sensitive fluorescence-based detection methods in cell biology and molecular biology studies.
• Diagnostic Kit Development: A key component in the formulation of diagnostic kits for clinical and research laboratories, enabling the detection of specific enzymatic biomarkers.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology drug discovery programs to screen for enzyme inhibitors or activators.
• Fluorescence Labeling and Sensing: Can be used as a precursor or intermediate for synthesizing more complex fluorescent probes and sensors for chemical or biological analytes.

Basic Information

Product Name	Fluorescein Di-p-Guanidinobenzoate Hydrochloride
CAS No.	135367-80-5
Molecular Formula	C46H38Cl2N8O8
Molecular Weight	877.75 g/mol
Synonyms	Fluorescein Di-p-Guanidinobenzoate HCl; FDGBA-HCl; Bis(p-guanidinobenzoyl)fluorescein Hydrochloride; Fluorescein Di(p-guanidinobenzoate) Dihydrochloride; Fluorescein Diguanidinobenzoate Hydrochloride; Protease Fluorogenic Substrate; Trypsin Substrate FDGBA
EINECS	Contact for details

Quality Control

Every batch of Fluorescein Di-p-Guanidinobenzoate Hydrochloride is manufactured and tested under a strict quality management system. We ensure high chemical purity and batch-to-batch consistency through advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with each shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.