Description

Fluo 3 is a high-purity, cell-permeant fluorescent calcium indicator widely used in biological research. This compound is critical for the quantitative measurement of intracellular calcium ion (Ca²⁺) concentrations in live cells, enabling real-time analysis of cellular signaling pathways. It is an essential tool for researchers and professionals in neuroscience, pharmacology, cell biology, and drug discovery who require precise and reliable fluorescent probes for imaging and assay development.

Application

• Intracellular Calcium Imaging: Primary use for visualizing and quantifying dynamic changes in cytosolic Ca²⁺ levels in live cells using fluorescence microscopy (e.g., confocal, epifluorescence).
• Neuroscience Research: Studying calcium signaling in neurons, including synaptic transmission, neuronal excitability, and activity-dependent processes.
• Drug Screening & Pharmacology: Employed in high-throughput screening (HTS) assays to evaluate the effects of pharmaceutical compounds on calcium-mediated cellular responses.
• Cardiovascular & Muscle Physiology: Monitoring calcium transients in cardiomyocytes, smooth muscle, and skeletal muscle cells to understand contractility and related disorders.
• Cell Signaling Pathway Analysis: Investigating G-protein coupled receptor (GPCR) activation, ion channel function, and second messenger systems linked to calcium influx.
• Apoptosis & Cell Viability Studies: Used as an indicator in assays measuring calcium flux associated with programmed cell death and cellular stress responses.

Basic Information

Item	Detail
Product Name	Fluo 3
CAS No.	134907-84-9
Molecular Formula	C₅₁H₅₀Cl₂N₂O₂₃
Molecular Weight	1129.85 g/mol
Synonyms	Fluo-3; Fluo 3 AM; Fluo-3 AM ester; Fluo-3, acetoxymethyl ester; Fluo-3, pentapotassium salt; 2-[6-[Bis(carboxymethyl)amino]-5-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid, AM ester; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, acetoxymethyl ester
EINECS	Contact for details

Quality Control

Our Fluo 3 is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and fluorescence validation. A detailed Certificate of Analysis (COA) providing purity, identity, and performance data is supplied with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Ex/Em ~506/526 nm (bound to Ca²⁺)
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.