Description

N(Alpha)-Pteroyl-N(Epsilon)-(4'-Fluoresceinthiocarbamoyl)Lysine is a sophisticated, dual-labeled chemical conjugate designed for advanced research applications. This compound matters because it combines the specific biochemical targeting of folic acid (pteroyl) with the high-sensitivity detection capability of fluorescein, creating a powerful tool for probing biological systems. It is primarily needed by researchers and developers in the fields of biochemistry, molecular biology, and diagnostic assay development who require precise, fluorescently tagged ligands for studies involving folate receptors or related metabolic pathways.

Application

• Biochemical Research Probe: Used as a high-affinity, fluorescent tracer for studying folate receptor (FR) binding, internalization, and cellular uptake mechanisms.
• Diagnostic Assay Development: Serves as a critical component in the development of sensitive fluorescence-based immunoassays (FLISA) and other in vitro diagnostic tests.
• Cellular Imaging: Enables visualization and tracking of folate-mediated processes in live or fixed cells using fluorescence microscopy and flow cytometry.
• Drug Delivery Research: Acts as a model compound for investigating targeted drug delivery systems that utilize the folate receptor pathway.
• Protein & Antibody Labeling: Can be used in specialized protocols for the fluorescent labeling of proteins, antibodies, or other biomolecules via its reactive lysine moiety.
• Competitive Binding Studies: Employed in quantitative assays to determine the binding affinity of other molecules for the folate receptor.

Basic Information

Product Name	N(Alpha)-Pteroyl-N(Epsilon)-(4'-Fluoresceinthiocarbamoyl)Lysine
CAS No.	133648-13-2
Molecular Formula	C41H38N8O9S
Molecular Weight	818.86 g/mol
Synonyms	Folate-Fluorescein Conjugate; Fluorescein-Folic Acid Conjugate; Pteroic acid-α-(N-fluoresceinthiocarbamoyl)lysine; Folic Acid-Fluorescein Isothiocyanate (FITC) Conjugate; Nα-Pteroyl-Nε-(fluorescein-5-thiocarbamoyl)-L-lysine; 5-FITC-Folic Acid; Fluorescein labeled Folic Acid; Folate-5-FITC
EINECS	Contact for details

Quality Control

Our N(Alpha)-Pteroyl-N(Epsilon)-(4'-Fluoresceinthiocarbamoyl)Lysine is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to meet exacting research standards. A comprehensive Certificate of Analysis (COA) detailing purity, spectral data, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the product to equilibrate to room temperature in a dry environment before opening to prevent condensation. The material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms to reference standard
Identification (UV-Vis)	Conforms to reference spectrum
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~68,000 cm⁻¹M⁻¹ at 494 nm (in buffer)
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.