Description

5(6)-Carboxyeosin For Fluorescence is a high-purity, reactive derivative of eosin, specifically designed as a versatile fluorescent labeling reagent. This compound is essential for creating stable, fluorescent conjugates with proteins, antibodies, and other biomolecules for advanced detection and imaging applications. It is a critical tool for researchers and manufacturers in the fields of life sciences, diagnostics, and biotechnology, enabling sensitive assays, flow cytometry, and fluorescence microscopy.

Application

• Fluorescent Labeling of Biomolecules: Primary application for covalently tagging proteins, peptides, and antibodies to create fluorescent probes.
• Immunofluorescence Assays (IFA): Used in the development of diagnostic kits and research assays for antigen detection.
• Flow Cytometry: Conjugation to antibodies for cell surface marker analysis and intracellular staining.
• Fluorescence Microscopy: Preparation of labeled tracers for cellular and tissue imaging studies.
• Bioconjugation & Probe Synthesis: A key intermediate in the synthesis of custom fluorescent probes and sensor molecules.
• Histology & In Situ Hybridization: Can be used in fluorescence in situ hybridization (FISH) and other histological staining techniques.

Basic Information

Product Name	5(6)-Carboxyeosin For Fluorescence
CAS No.	132201-84-4
Molecular Formula	C22H11Br4O7
Molecular Weight	691.84 g/mol
Synonyms	5(6)-Carboxyeosin; Eosin-5-isothiocyanate precursor; 2',4',5',7'-Tetrabromo-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5(or 6)-carboxylic acid; Carboxyeosin; Eosin carboxylic acid; Reactive Eosin derivative; Fluorescent labeling reagent Eosin
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxyeosin For Fluorescence is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the stringent requirements for fluorescence labeling applications. A Certificate of Analysis (COA) detailing purity, absorbance, fluorescence properties, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is light-sensitive (store away from light). For long-term storage, consider desiccation to maintain stability.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Fluorescence Grade)
Absorbance (λmax)	~525-535 nm
Fluorescence Emission (λmax)	~545-555 nm
Loss on Drying	≤ 2.0 %
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.