Description

2,5-Dioxopyrrolidin-1-Yl Pent-4-Ynoate is a specialized active ester reagent used in organic synthesis and bioconjugation chemistry. Its core value lies in its ability to efficiently couple carboxylic acids to amines under mild conditions, forming stable amide bonds. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and advanced materials sectors who require high-purity, reliable building blocks for peptide synthesis, protein labeling, and polymer functionalization.

Application

• Peptide Synthesis: Acts as a key coupling agent for the formation of amide bonds in solid-phase and solution-phase peptide synthesis.
• Bioconjugation & Protein Labeling: Used to attach fluorescent dyes, affinity tags (e.g., biotin), or other functional molecules to proteins, antibodies, and other biomolecules.
• Polymer & Material Science: Functionalizes polymer chains or material surfaces with alkyne groups for subsequent "click chemistry" reactions, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• Small Molecule Drug Development: Serves as an intermediate in the synthesis of more complex active pharmaceutical ingredients (APIs) and prodrugs.
• Cross-linking Agent: Utilized to create cross-linked hydrogels, polymer networks, or immobilized enzyme preparations.
• Chemical Probe Synthesis: Enables the creation of activity-based probes for studying enzyme function and target engagement in biological systems.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl Pent-4-Ynoate
CAS No.	132178-37-1
Molecular Formula	C9H9NO4
Molecular Weight	195.17 g/mol
Synonyms	Pent-4-ynoic acid 2,5-dioxo-1-pyrrolidinyl ester; 4-Pentynoic acid N-hydroxysuccinimide ester; NHS-Pentynoate; N-Hydroxysuccinimide ester of 4-pentynoic acid; 4-Pentynoic acid succinimidyl ester; 4-Pentynoyloxy succinimide; 4-Pentynoic acid, 2,5-dioxo-1-pyrrolidinyl ester; Alkyne-NHS ester
EINECS	Contact for details

Quality Control

Our 2,5-Dioxopyrrolidin-1-Yl Pent-4-Ynoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistency, meeting the stringent requirements for research and process chemistry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.